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|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Mink, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Mouse recombinant p27 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12832 targets p27Kip1 in FACS, IF, IP, and WB applications and shows reactivity with Canine, Human, mouse, and Rat samples.
The MA5-12832 immunogen is mouse recombinant p27 protein.
p27Kip1, a candidate tumor suppressor gene, functions as a negative regulator of G1 progression and has been proposed to fuction as a possible mediator of TGF-beta induced G1 arrest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Apigenin inhibits prostate cancer progression in TRAMP mice via targeting PI3K/Akt/FoxO pathway.
MA5-12832 was used in immunoprecipitation and western blot to study the role of PI3 kinase/Akt/FoxO signaling in the mechanism by which apigenin inhibits the progression of prostate cancer in a murine model
|Shukla S,Bhaskaran N,Babcook MA,Fu P,Maclennan GT,Gupta S||Carcinogenesis (35:452)||2014|
|Not Applicable||Not Cited||
Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.
MA5-12832 was used in immunoprecipitation to study if the growth state-dependent tyrosine phosphorylation of p27Kip1 determines whether or not it inhibits cdk4
|James MK,Ray A,Leznova D,Blain SW||Molecular and cellular biology (28:498)||2008|
MicroRNA-223 regulates FOXO1 expression and cell proliferation.
MA5-12832 was used in western blot to study the effects of microRNA-223 overexpression on the expression of FOXO1 and on cell proliferation
|Wu L,Li H,Jia CY,Cheng W,Yu M,Peng M,Zhu Y,Zhao Q,Dong YW,Shao K,Wu A,Wu XZ||FEBS letters (586:1038)||2012|
MiR-223 suppresses cell proliferation by targeting IGF-1R.
MA5-12832 was used in western blot to study the mechanism by which microRNA-223 attenuates cell proliferation
|Jia CY,Li HH,Zhu XC,Dong YW,Fu D,Zhao QL,Wu W,Wu XZ||PloS one (6:null)||2011|
Penta-O-galloyl-beta-D-glucose induces S- and G(1)-cell cycle arrests in prostate cancer cells targeting DNA replication and cyclin D1.
MA5-12832 was used in western blot to examine the effect of penta-O-galloyl-beta-D-glucose on prostate cancer cell proliferation
|Hu H,Zhang J,Lee HJ,Kim SH,Lü J||Carcinogenesis (30:818)||2009|
Progressive changes in hepatoma cells stably transfected with hepatitis B virus X gene.
MA5-12832 was used in western blot to study the molecular mechanism of hepatocellular carcinoma development induced by hepatitis B virus X protein
|Ye L,Dong N,Wang Q,Xu Z,Cai N,Wang H,Zhang X||Intervirology (51:50)||2008|
BAF180 is a critical regulator of p21 induction and a tumor suppressor mutated in breast cancer.
MA5-12832 was used in western blot to study the role of BAF180 in regulating p21 expression in breast cancer
|Xia W,Nagase S,Montia AG,Kalachikov SM,Keniry M,Su T,Memeo L,Hibshoosh H,Parsons R||Cancer research (68:1667)||2008|
Disruption of heparan and chondroitin sulfate signaling enhances mesenchymal stem cell-derived osteogenic differentiation via bone morphogenetic protein signaling pathways.
MA5-12832 was used in western blot to study the role of heparan and chondroitin sulfate signaling in the osteogenic differentiation of mesenchymal stem cells
|Manton KJ,Leong DF,Cool SM,Nurcombe V||Stem cells (Dayton, Ohio) (25:2845)||2007|
Antitumor activity of CTFB, a novel anticancer agent, is associated with the down-regulation of nuclear factor-kappaB expression and proteasome activation in head and neck squamous carcinoma cell lines.
MA5-12832 was used in western blot to investigate the mechanism for the antitumor activity of 5-Chloro-N-[2-[2-(4-chloro-phenyl)-3-methyl-butoxy]-5-trifluoromethyl-phenyl]-2-hydroxy-benzamide in head and neck cancer cell lines
|Skvortsov S,Skvortsova I,Stasyk T,Schiefermeier N,Neher A,Gunkel AR,Bonn GK,Huber LA,Lukas P,Pleiman CM,Zwierzina H||Molecular cancer therapeutics (6:1898)||2007|
Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells.
MA5-12832 was used in western blot to study the role of p21(Cip1) and p27(Kip1) in the induction of cell cycle arrest in human panceatic adenocarcinoma cells by isoprenoids
|Wiseman DA,Werner SR,Crowell PL||The Journal of pharmacology and experimental therapeutics (320:1163)||2007|
N-all-trans-retinoyl-L-proline inhibits metastatic potential of hepatocellular carcinoma cells.
MA5-12832 was used in western blot to examine the therapeutic effect of N-all-trans-retinoyl-L-proline in the metastasis of hepatocellular carcinoma cells
|Wu XZ,Shi PC,Hu P,Chen Y,Ding SS||Cell biology international (30:672)||2006|
Iressa induces cytostasis and augments Fas-mediated apoptosis in acinic cell adenocarcinoma overexpressing HER2/neu.
MA5-12832 was used in western blot to study the mechanism by which Iressa induces cytostasis and augments Fas-mediated apoptosis in acinic cell adenocarcinoma
|Piechocki MP,Yoo GH,Dibbley SK,Amjad EH,Lonardo F||International journal of cancer (119:441)||2006|
E Proteins and Id2 converge on p57Kip2 to regulate cell cycle in neural cells.
MA5-12832 was used in western blot to study the role of E-protein transcription factors and Id2 in cell cycle regulation in neural cells
|Rothschild G,Zhao X,Iavarone A,Lasorella A||Molecular and cellular biology (26:4351)||2006|
Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells.
MA5-12832 was used in western blot to investigate the effect of valproate on Ara-C cytotoxicity in acute myeloblastic leukemia cells and its mechanism
|Siitonen T,Koistinen P,Savolainen ER||Leukemia research (29:1335)||2005|
An in vivo evaluation of docetaxel delivered intratumorally in head and neck squamous cell carcinoma.
MA5-12832 was used in western blot to study the mechanisms of action of intra-tumoral docetaxel in head and neck squamous cell carcinoma
|Yoo GH,Subramanian G,Boinpally RR,Iskander A,Shehadeh N,Oliver J,Ezzat W,Piechocki MP,Ensley JF,Lin HS,Shibuya TY,Polin L,Parchment RE||Archives of otolaryngology--head & neck surgery (131:418)||2005|
Silibinin protects against photocarcinogenesis via modulation of cell cycle regulators, mitogen-activated protein kinases, and Akt signaling.
MA5-12832 was used in western blot to investigate the mechanism of photocarcinogenesis prevention by silibinin
|Mallikarjuna G,Dhanalakshmi S,Singh RP,Agarwal C,Agarwal R||Cancer research (64:6349)||2004|
Insulin-like growth factor-I receptor signaling and resistance to trastuzumab (Herceptin).
MA5-12832 was used in western blot to study whether IGF-I interferes with the growth-inhibitory action of trastuzumab in breast cancer cells
|Lu Y,Zi X,Zhao Y,Mascarenhas D,Pollak M||Journal of the National Cancer Institute (93:1852)||2001|
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
MA5-12832 was used in western blot to study the role of CDK4 in cell cycle regulation using targeted knockdown
|Tsutsui T,Hesabi B,Moons DS,Pandolfi PP,Hansel KS,Koff A,Kiyokawa H||Molecular and cellular biology (19:7011)||1999|
Inflammatory mediators of esophagitis alter p27 Kip1 expression in esophageal epithelial cells.
MA5-12832 was used in immunocytochemistry to study the role of inflammatory mediators of esophagitis in altered p27(Kip1) expression in esophageal epithelial cells
|Nguyen KD,Blain SW,Gress F,Treem WR||Journal of pediatric gastroenterology and nutrition (51:556)||2010|
In vitro growth and differentiation of mammalian sensory hair cell progenitors: a requirement for EGF and periotic mesenchyme.
MA5-12832 was used in immunocytochemistry to evaluate an invitro cell culture system for the study of mammalian sensory hair cell growth and differentiation
|Doetzlhofer A,White PM,Johnson JE,Segil N,Groves AK||Developmental biology (272:432)||2004|
p27Kip1 localizes to detergent-insoluble microdomains within lymphocyte membranes.
MA5-12832 was used in immunocytochemistry to study the presence and significance of p27Kip1 in detergent-insoluble microdomains within lymphocyte membranes
|Yaroslavskiy BB,Stolz DB,Watkins SC,Alber SM,Bradbury NA,Steinman RA||Molecular medicine (Cambridge, Mass.) (7:49)||2001|
The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2.
MA5-12832 was used in immunocytochemistry to study the role of p15(Ink4b) and p27(Kip1) subcellular localization in the coordinate regulation of cdk2 and cdk4
|Reynisdóttir I,Massagué J||Genes & development (11:492)||1997|
Presence of HER4 associates with increased sensitivity to Herceptin in patients with metastatic breast cancer.
MA5-12832 was used in immunohistochemistry to investigate the mechanism for cellular susceptibility to Herceptin
|Sassen A,Diermeier-Daucher S,Sieben M,Ortmann O,Hofstaedter F,Schwarz S,Brockhoff G||Breast cancer research : BCR (11:null)||2009|
Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis.
MA5-12832 was used in immunohistochemistry to study aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma
|Zhang W,Zeng Z,Zhou Y,Xiong W,Fan S,Xiao L,Huang D,Li Z,Li D,Wu M,Li X,Shen S,Wang R,Cao L,Tang K,Li G||Acta biochimica et biophysica Sinica (41:414)||2009|
Upregulation of TNF receptor type 2 in human and experimental renal allograft rejection.
MA5-12832 was used in immunohistochemistry to study the expression and potential role of TNFR2 in human and experimental renal allograft rejection
|Hoffmann U,Bergler T,Rihm M,Pace C,Krüger B,Rümmele P,Stoelcker B,Banas B,Männel DN,Krämer BK||American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons (9:675)||2009|
Activated Akt as an indicator of prognosis in gastric cancer.
MA5-12832 was used in immunohistochemistry to examine the prognostic significance of activated AKT in gastric cancer
|Cinti C,Vindigni C,Zamparelli A,La Sala D,Epistolato MC,Marrelli D,Cevenini G,Tosi P||Virchows Archiv : an international journal of pathology (453:449)||2008|
The combination of high cyclin E and Skp2 expression in breast cancer is associated with a poor prognosis and the basal phenotype.
MA5-12832 was used in immunohistochemistry to study the prognostic value of high expression of both cyclin E and Skp2 in basal phenotype breast cancer
|Voduc D,Nielsen TO,Cheang MC,Foulkes WD||Human pathology (39:1431)||2008|
Expression of senescence-related genes in human corneal endothelial cells.
MA5-12832 was used in immunohistochemistry to study the expression of senescence-related genes in human corneal endothelial cells from donors of different ages
|Song Z,Wang Y,Xie L,Zang X,Yin H||Molecular vision (14:161)||2008|
Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry.
MA5-12832 was used in immunohistochemistry to evaluate different fixation and antigen retrieval methods for immmunohistochemical studies on frozen tissues
|Shi SR,Liu C,Pootrakul L,Tang L,Young A,Chen R,Cote RJ,Taylor CR||American journal of clinical pathology (129:358)||2008|
Prognostic implications of cyclin B1, p34cdc2, p27(Kip1) and p53 expression in gastric cancer.
MA5-12832 was used in immunohistochemistry to study the prognostic significance of the expression of cyclin B1, p34cdc2, p27(Kip1) and p53 in gastric cancer
|Kim DH||Yonsei medical journal (48:694)||2007|
Increased expression of CCAAT/enhancer-binding protein beta in proliferative inflammatory atrophy of the prostate: relation with the expression of COX-2, the androgen receptor, and presence of focal chronic inflammation.
MA5-12832 was used in immunohistochemistry to study the mechanisms driving increased expression of C/EBPbeta in proliferative inflammatory atrophy of the prostate
|Wang W,Bergh A,Damber JE||The Prostate (67:1238)||2007|
Increased SKP2 and CKS1 gene expression contributes to the progression of human urothelial carcinoma.
MA5-12832 was used in immunohistochemistry to study the role of SKP2 and CKS1 in the progression of human urothelial carcinoma
|Kawakami K,Enokida H,Tachiwada T,Nishiyama K,Seki N,Nakagawa M||The Journal of urology (178:301)||2007|
Prospective identification and purification of hair cell and supporting cell progenitors from the embryonic cochlea.
MA5-12832 was used in immunohistochemistry to investigate the progenitors of hair cells and supporting cells from embryonic mouse cochlea
|Doetzlhofer A,White P,Lee YS,Groves A,Segil N||Brain research (1091:282)||2006|
Suppressor of cytokine signalling 2 (SOCS-2) expression in breast carcinoma.
MA5-12832 was used in immunohistochemistry to study SOCS2 expression and survival in breast carcinoma
|Farabegoli F,Ceccarelli C,Santini D,Taffurelli M||Journal of clinical pathology (58:1046)||2005|
Expression of p8 protein in breast carcinoma; an inverse relationship with apoptosis.
MA5-12832 was used in immunohistochemistry to study the expression of p8 protein in breast carcinoma and its relationship with apoptosis
|Ito Y,Yoshida H,Motoo Y,Iovanna JL,Nakamura Y,Kakudo K,Uruno T,Takamura Y,Miya A,Noguchi S,Kuma K,Miyauchi A||Anticancer research (25:833)||2005|
Expression of Islet1 marks the sensory and neuronal lineages in the mammalian inner ear.
MA5-12832 was used in immunohistochemistry to study Islet1 expression as a marker of sensory and neuronal cell lineage in the mammalian inner ear
|Radde-Gallwitz K,Pan L,Gan L,Lin X,Segil N,Chen P||The Journal of comparative neurology (477:412)||2004|
Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.
MA5-12832 was used in immunohistochemistry to evaluate immunohistochemical markers of non-small cell lung cancer using unsupervised hierarchical clustering analysis
|Au NH,Cheang M,Huntsman DG,Yorida E,Coldman A,Elliott WM,Bebb G,Flint J,English J,Gilks CB,Grimes HL||The Journal of pathology (204:101)||2004|
Predicting early failure after adjuvant chemotherapy in high-risk breast cancer patients with extensive lymph node involvement.
MA5-12832 was used in immunohistochemistry to study the relationship between early failure after adjuvant chemotherapy and poor survival in high-risk breast cancer
|Faneyte IF,Peterse JL,Van Tinteren H,Pronk C,Bontenbal M,Beex LV,van der Wall E,Richel DJ,Nooij MA,Voest EE,Hupperets P,Ten Vergert EM,de Vries EG,Rodenhuis S,van de Vijver MJ||Clinical cancer research : an official journal of the American Association for Cancer Research (10:4457)||2004|
Significantly decreased P27 expression in endometrial carcinoma compared to complex hyperplasia with atypia (correlation with p53 expression).
MA5-12832 was used in immunohistochemistry to study p27 and p53 expression in endometrial carcinoma compared to complex hyperplasia with atypia
|Ozkara SK,Corakci A||Pathology oncology research : POR (10:89)||2004|
pRb2/p130, vascular endothelial growth factor, p27(KIP1), and proliferating cell nuclear antigen expression in hepatocellular carcinoma: their clinical significance.
MA5-12832 was used in immunohistochemistry to study the expression of pRb2/p130, VEGF, p27(KIP1), and PCNA as potential markers of hepatocellular carcinoma
|Claudio PP,Russo G,Kumar CA,Minimo C,Farina A,Tutton S,Nuzzo G,Giuliante F,Angeloni G,Maria V,Vecchio FM,Campli CD,Giordano A||Clinical cancer research : an official journal of the American Association for Cancer Research (10:3509)||2004|
Simultaneous chromosome 1q gain and 16q loss is associated with steroid receptor presence and low proliferation in breast carcinoma.
MA5-12832 was used in immunohistochemistry to study the association of simultaneous chromosome 1q gain and 16q loss with low proliferation in breast carcinoma
|Farabegoli F,Hermsen MA,Ceccarelli C,Santini D,Weiss MM,Meijer GA,van Diest PJ||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (17:449)||2004|
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
MA5-12832 was used in immunohistochemistry to investigate the influence of Bcl-2 reduction on the progression and prognosis of human IgA nephropathy
|Qiu LQ,Sinniah R,I-Hong Hsu S||Journal of the American Society of Nephrology : JASN (15:79)||2004|
Improved endometrial assessment using cyclin E and p27.
MA5-12832 was used in immunohistochemistry to evaluate cyclin E and p27 as developmental markers for endometrium
|Dubowy RL,Feinberg RF,Keefe DL,Doncel GF,Williams SC,McSweet JC,Kliman HJ||Fertility and sterility (80:146)||2003|
Expression of cell-cycle-regulated proteins pRb2/p130, p107, p27(kip1), p53, mdm-2, and Ki-67 (MIB-1) in prostatic gland adenocarcinoma.
MA5-12832 was used in immunohistochemistry to study the expression and prognostic value of cell-cycle-regulated proteins in prostatic gland adenocarcinoma
|Claudio PP,Zamparelli A,Garcia FU,Claudio L,Ammirati G,Farina A,Bovicelli A,Russo G,Giordano GG,McGinnis DE,Giordano A,Cardi G||Clinical cancer research : an official journal of the American Association for Cancer Research (8:1808)||2002|
The role of Math1 in inner ear development: Uncoupling the establishment of the sensory primordium from hair cell fate determination.
MA5-12832 was used in immunohistochemistry to investigate the role of Math1 in the development of hair cells in inner ear
|Chen P,Johnson JE,Zoghbi HY,Segil N||Development (Cambridge, England) (129:2495)||2002|
p27Kip1 expression is associated with clinical outcome in advanced epithelial ovarian cancer: multivariate analysis.
MA5-12832 was used in immunohistochemistry to investigate the prognostic significance of the p27(Kip1) in advanced epithelial ovarian cancer
|Masciullo V,Ferrandina G,Pucci B,Fanfani F,Lovergine S,Palazzo J,Zannoni G,Mancuso S,Scambia G,Giordano A||Clinical cancer research : an official journal of the American Association for Cancer Research (6:4816)||2000|
cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); Cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor p27; cyclin-dependent kinase inhibitor p27/Kip1; putative cyclin-dependent kinase inhibitor p27
AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1