Immunohistochemistry analysis of p27Kip1 showing staining in the nucleus of paraffin-embedded human prostate tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p27Kip1 Rabbit Polyclonal Antibody (PA516717) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminal of human p27Kip1|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 8 publications below|
PA5-16717 targets p27Kip1 in IHC (P) applications and shows reactivity with Human, Mouse and Rat samples.
The PA5-16717 immunogen is synthetic peptide derived from C-terminal of human p27Kip1.
Recognizes a protein of 27kDa, identified as p27Kip1. p27Kip1 is a candidate tumor suppressor gene and functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest.
IP-MS enrichment of p27 Kip1 (LFQ intensity): p27 Kip1 was enriched 5386-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and p27 Kip1 antibody (Part No. PA5-16717). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The expression of cell cycle related proteins PCNA, Ki67, p27 and p57 in normal and preeclamptic human placentas.
PA5-16717 was used in immunohistochemistry to examine human placentas from normal and preeclampsic pregnancies for the expression of PCNA, Ki67 and p57
|Unek G,Ozmen A,Mendilcioglu I,Simsek M,Korgun ET||Tissue and cell (46:198)||2014|
Immunolocalization of cell cycle proteins (p57, p27, cyclin D3, PCNA and Ki67) in intrauterine growth retardation (IUGR) and normal human term placentas.
PA5-16717 was used in immunohistochemistry to perform a comparative study of intrauterine growth retardation and normal human term placentas in respect of the expression and localization of p57, p27, cyclin D3, PCNA and Ki67
|Unek G,Ozmen A,Ozekinci M,Sakinci M,Korgun ET||Acta histochemica (116:493)||2014|
Immunohistochemical distribution of cell cycle proteins p27, p57, cyclin D3, PCNA and Ki67 in normal and diabetic human placentas.
PA5-16717 was used in immunohistochemistry to examine human placentas from control and diabetic subjects for the immunohistochemical localization of a number of cell cycle related proteins
|Unek G,Ozmen A,Mendilcioglu I,Simsek M,Korgun ET||Journal of molecular histology (45:21)||2014|
The diagnostic value of p27 in comparison to p57 in differentiation between different gestational trophoblastic diseases.
PA5-16717 was used in immunohistochemistry to study the relative diagnostic value of p27 and p57 in gestational trophoblastic diseases
|Abdou A,Kandil M,El-Wahed MA,Shabaan M,El-Sharkawy M||Fetal and pediatric pathology (32:395)||2013|
Expression of insulin-like growth factor binding proteins during mouse cochlear development.
PA5-16717 was used in immunohistochemistry to study the expression of IGFBP family members during development of the murine cochlear
|Okano T,Kelley MW||Developmental dynamics : an official publication of the American Association of Anatomists (242:1210)||2013|
Expression of anti-Müllerian hormone, CDKN1B, connexin 43, androgen receptor and steroidogenic enzymes in the equine cryptorchid testis.
PA5-16717 was used in immunohistochemistry to perform a comparative study of the expression of AMH, AMHR2, CDKN1B, connexin-43, AR and enzymes involved in steroidogenesis in testes from control and cryptorchid horses
|Almeida J,Conley AJ,Ball BA||Equine veterinary journal (45:538)||2013|
Expression of p27, COX-2, MLH1, and MSH2 in young patients with colon carcinoma and correlation with morphologic findings.
PA5-16717 was used in immunohistochemistry to study colon carcinoma in young patients and the relationship between morphology and the expression of a range of prognostic and therapeutic markers
|Kenney B,Deng Y,Mitchell K||Human pathology (44:591)||2013|
Immunolocalization of PCNA, Ki67, p27 and p57 in normal and dexamethasone-induced intrauterine growth restriction placental development in rat.
PA5-16717 was used in immunohistochemistry to study localization of PCNA, Ki67, p27 and p57 in normal and dexamethasone-induced IUGR placental development in rats
|Unek G,Ozmen A,Kipmen-Korgun D,Korgun ET||Acta histochemica (114:31)||2012|