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|Tested species reactivity||Human, Mustelid, Mouse, Primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Fusion protein containing residues 1921-2023 of human p300.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Western Blot (WB)||1:250-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Suggested positive control: HeLa nuclear extract.
p300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which is conserved among a variety of mammalian species. It is actively synthesized and phosphorylated in both quiescent and proliferating cells. Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators and have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes. As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reactive oxygen species, AMP-activated protein kinase, and the transcription cofactor p300 regulate α-tubulin acetyltransferase-1 (αTAT-1/MEC-17)-dependent microtubule hyperacetylation during cell stress.
MA1-16622 was used in western blot to study mechanisms of microtubule hyperacetylation
|Mackeh R,Lorin S,Ratier A,Mejdoubi-Charef N,Baillet A,Bruneel A,Hamaï A,Codogno P,Poüs C,Perdiz D||The Journal of biological chemistry (289:11816)||2014|
Cyclic AMP responsive enhancer binding protein; E1A-associated protein p300; E1A-binding protein, 300kD; EP300: E1A binding protein p300; histone acetyltransferase p300; p300 HAT; RB and P300 binding protein EID 1
A430090G16; A730011L11; EP300; KAT3B; P300; p300 HAT; RSTS2