Invitrogen

p300 Monoclonal Antibody (NM-11)

Advanced Verification

This Antibody was verified by IP Mass Spectrometry to ensure that the antibody binds to the antigen stated. View Details

View all (14) p300 antibodies

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p300 Antibody in IP-MS — IP-MS enrichment of EP300 (LFQ intensity): EP300 was enriched 666-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and EP300 antibody (Product # 33-7600). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

p300 Antibody in Western Blot (WB) — Western blot analysis of HeLa cell lysates using p300 Monoclonal Antibody, Mouse/CBP (Product # 33-7600)

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (IHC)

Assay Dependent

Immunoprecipitation (IP)

Assay Dependent

Western Blot (WB)

Assay Dependent

Published Applications

Flow Cytometry (Flow)

See 1 publication below

Western Blot (WB)

See 3 publications below

Immunoprecipitation (IP)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

NM-11

Immunogen

Recombinant human p300 protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533138

Target Information

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: E1A-associated protein p300; E1A-binding protein, 300kD; EP300; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; KAT3B; p300 HAT; Protein propionyltransferase p300

Gene Aliases: EP300; KAT3B; P300; RSTS2

UniProt ID: (Human) Q09472

Entrez Gene ID: (Human) 2033

Molecular Function: DNA binding protein acetyltransferase chromatin/chromatin-binding protein nucleic acid binding transcription cofactor transcription factor transferase

negative regulation of transcription from RNA polymerase II promoter response to hypoxia somitogenesis liver development positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway transcription-coupled nucleotide-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein acetylation internal protein amino acid acetylation apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Notch signaling pathway nervous system development heart development skeletal muscle tissue development memory circadian rhythm organ morphogenesis regulation of autophagy positive regulation of glycoprotein biosynthetic process macrophage derived foam cell differentiation positive regulation of cell death positive regulation of muscle atrophy viral process N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation B cell differentiation platelet formation lung development response to cobalt ion positive regulation of protein binding positive regulation of type I interferon production positive regulation of collagen biosynthetic process positive regulation of protein import into nucleus, translocation response to tumor necrosis factor cellular response to UV positive regulation of histone acetylation cellular response to drug megakaryocyte development cellular response to trichostatin A intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of DNA binding protein kinase B signaling response to estrogen positive regulation by host of viral transcription histone H3 acetylation histone H4 acetylation histone H2B acetylation fat cell differentiation response to ethanol positive regulation of translation positive regulation of axon extension positive regulation of cell size positive regulation of gene expression, epigenetic positive regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter digestive tract development positive regulation of protein secretion protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity response to calcium ion regulation of cell cycle regulation of angiotensin metabolic process positive regulation of sarcomere organization regulation of androgen receptor signaling pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia protein-DNA complex assembly cellular response to hydrogen peroxide response to fatty acid cellular response to retinoic acid cellular response to cAMP cellular response to glucose stimulus cellular response to mineralocorticoid stimulus cellular response to dexamethasone stimulus regulation of tubulin deacetylation regulation of cellular response to heat regulation of signal transduction by p53 class mediator beta-catenin-TCF complex assembly cellular response to nerve growth factor stimulus negative regulation of miRNA metabolic process

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding chromatin binding damaged DNA binding transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor protein binding beta-catenin binding protein C-terminus binding transcription factor binding zinc ion binding acetyltransferase activity transferase activity, transferring acyl groups chromatin DNA binding protein complex binding activating transcription factor binding peptide N-acetyltransferase activity nuclear hormone receptor binding glucocorticoid receptor binding peroxisome proliferator activated receptor binding bHLH transcription factor binding SMAD binding androgen receptor binding mitogen-activated protein kinase binding NF-kappaB binding pre-mRNA intronic binding protein antigen binding

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