Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:250-1:500 | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
2-5 µg/mL | - |
Immunoprecipitation (IP) |
1:10-1:500 | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mustelid, Mouse, Non-human primate, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Mouse / IgG1, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
RW128 |
Immunogen |
Fusion protein containing residues 1572-2371 of human p300. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4 |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_568667 |
Suggested positive control: HeLa nuclear extract.
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E1A-associated protein p300; E1A-binding protein, 300kD; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300
Gene Aliases: A430090G16; A730011L11; EP300; KAT3B; P300; p300 HAT; RSTS2
UniProt ID: (Human) Q09472, (Mouse) B2RWS6
Entrez Gene ID: (Human) 2033, (Mouse) 328572, (Rat) 170915
Molecular Function:
histone modifying enzyme
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