|Tested species reactivity||Bovine, Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Bacterially expressed full-length human Cdk1 protein|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||10µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody will not cross-react with Drosophila, mouse, Xenopus or rat.
Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Biological dose estimation of UVA laser microirradiation utilizing charged particle-induced protein foci.
MA1-19057 was used in immunocytochemistry to detect irradiation dose using charged particle-induced protein foci
|Splinter J,Jakob B,Lang M,Yano K,Engelhardt J,Hell SW,Chen DJ,Durante M,Taucher-Scholz G||Mutagenesis (25:289)||2010|
Synthesis and structure-activity relationships of N-6 substituted analogues of 9-hydroxy-4-phenylpyrrolo[3,4-c]carbazole-1,3(2H,6H)-diones as inhibitors of Wee1 and Chk1 checkpoint kinases.
MA1-19057 was used in western blot to study the synthesis and SAR of novel small molecule inhibitors of the Wee1 and Chk1 checkpoint kinases
|Smaill JB,Baker EN,Booth RJ,Bridges AJ,Dickson JM,Dobrusin EM,Ivanovic I,Kraker AJ,Lee HH,Lunney EA,Ortwine DF,Palmer BD,Quin J,Squire CJ,Thompson AM,Denny WA||European journal of medicinal chemistry (43:1276)||2008|
Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.
MA1-19057 was used in western blot to examine the nuclear transport of caldsmon and its role in nuclear function
|Zheng PP,Weiden Mv,Sillevis Smitt PA,Luider TM,Kros JM||Cancer biology and therapy (6:886)||2007|
Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.
MA1-19057 was used in western blot to study the antiproliferative and apoptotic effects of ixocarpalactone A on colon cancer cells
|Choi JK,Murillo G,Su BN,Pezzuto JM,Kinghorn AD,Mehta RG||The FEBS journal (273:5714)||2006|
c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells.
MA1-19057 was used in western blot to study the effect of c-Myc on DNA damage-induced G2/M arrest.
|Sheen JH,Woo JK,Dickson RB||British journal of cancer (89:1479)||2003|