Immunofluorescent analysis of p38-alpha (green) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a mouse p38 monoclonal antibody (Product # 33-1300) at a dilution of 20ug/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27027) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Non-human primate, Rat, Human, Mouse|
|Published species reactivity||Rabbit, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Full-length recombinant mouse p38 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunocytochemistry (ICC)||5-20 ug/ml|
|Immunofluorescence (IF)||5-20 ug/ml|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
33-1300 has been successfully used in immunofluorescence, and western blot with human recombinant proteins and whole cell lysates of hamster, mouse, and primate origins.
33-1300 specifically recognizes both alpha and beta isoforms of p38, with slight reactivity to p38-delta.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases, or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Simulated Microgravity Disrupts Cytoskeleton Organization and Increases Apoptosis of Rat Neural Crest Stem Cells Via Upregulating CXCR4 Expression and RhoA-ROCK1-p38 MAPK-p53 Signaling.
33-1300 was used in immunocytochemistry and western blot to elucidate an increase of apoptosis and disruption of cytoskeleton organization of rat neural crest stem cells via upregulating CXCR4 expression and RhoA-ROCK1-p38 MAPK-p53 signaling due to simul
|Lin SC,Gou GH,Hsia CW,Ho CW,Huang KL,Wu YF,Lee SY,Chen YH||Stem cells and development (25:1172)||2016|
Translational control of inducible nitric oxide synthase by p38 MAPK in islet ß-cells.
33-1300 was used in western blot to test if MAPKs regulates posttranscriptional events in β-cells and primary islets in response to cytokine signaling.
|Nishiki Y,Adewola A,Hatanaka M,Templin AT,Maier B,Mirmira RG||Molecular endocrinology (Baltimore, Md.) (27:336)||2013|
|Rabbit||1:500||Stress kinase phosphorylation is increased in pacing-induced heart failure in rabbits.||Schulz R,Aker S,Belosjorow S,Konietzka I,Rauen U,Heusch G||American journal of physiology. Heart and circulatory physiology (285:H2084)||2003|
|Human||Not Cited||p38 mitogen-activated protein kinase is activated and linked to TNF-alpha signaling in inflammatory bowel disease.||Waetzig GH,Seegert D,Rosenstiel P,Nikolaus S,Schreiber S||Journal of immunology (Baltimore, Md. : 1950) (168:5342)||2002|
||Stress kinase phosphorylation is increased in pacing-induced heart failure in rabbits.||Schulz R,Aker S,Belosjorow S,Konietzka I,Rauen U,Heusch G||American journal of physiology. Heart and circulatory physiology (285:H2084)||2003|
||p38 mitogen-activated protein kinase is activated and linked to TNF-alpha signaling in inflammatory bowel disease.||Waetzig GH,Seegert D,Rosenstiel P,Nikolaus S,Schreiber S||Journal of immunology (Baltimore, Md. : 1950) (168:5342)||2002|