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Immunofluorescent analysis of p38-alpha (green) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a mouse p38 monoclonal antibody (Product # 33-1300) at a dilution of 20ug/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27027) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Full-length recombinant mouse p38 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunocytochemistry (ICC)||5-20 ug/ml|
|Immunofluorescence (IF)||5-20 ug/ml|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
33-1300 has been successfully used in immunofluorescence, and western blot with human recombinant proteins and whole cell lysates of hamster, mouse, and primate origins.
33-1300 specifically recognizes both alpha and beta isoforms of p38, with slight reactivity to p38-delta.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases, or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Translational control of inducible nitric oxide synthase by p38 MAPK in islet β-cells.
33-1300 was used in western blot to test if MAPKs regulates posttranscriptional events in β-cells and primary islets in response to cytokine signaling.
|Nishiki Y,Adewola A,Hatanaka M,Templin AT,Maier B,Mirmira RG||Molecular endocrinology (Baltimore, Md.) (27:336)||2013|
CRK1; CSAID binding protein; CSAID-binding protein; Csaids binding protein; CSBP1; CSBP2; Cytokine suppressive anti- inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; EC 184.108.40.206; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activ; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; MK14; MXI2; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; SAPK2a; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38
CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38-alpha; p38a; p38ALPHA; p38Hog; p38MAPK; PRKM14; PRKM15; RK; SAPK2A