A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus MAPK14 siRNA (Lane 3). Western blot data for Cycolphilin B is included as a control for equal protein loading.
|Tested species reactivity||Human, Mouse, Non-human primate, Pig, Rat, Yeast|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant mitogen-activated protein kinase 14|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
82382 targets MAPK14 in Western blot, immunofluorescence, immunocytochemistry, and flow cytometry applications and shows reactivity with human, mouse, monkey, pig, S. cerevisiae, and rat samples. 82382 does not cross-react with either JNK/SAPK or p42/44 MAP Kinase. MAPK14 has a predicted molecular weight of approximately 43 kDa.
Store at -20°C and do not aliquot.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.