Immunofluorescence analysis of p38-beta 2 MAPK Antibody (P38-11A5) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with p38-beta 2 MAPK Antibody (P38-11A5)(338700) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rabbit, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1/IgG2a|
|Immunogen||Recombinant, full-length human p38 beta-2; the product of the p38-beta gene|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
33-8700 has been successfully used in immunofluorescence and western blot.
33-8700 specifically recognizes the beta isoform of p38, with slight reactivity to p38-gamma.
MAPK11 is a Ser/Thr kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. It preferentially phosphorylates transcription factor ATF2. This protein is activated by phosphorylation on threonine and tyrosine by MKK6, and is inhibited by pyridinyl-imidazole related compounds. Highest expression levels are in the brain and heart, with additional expression in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Epithelial Control of Gut-Associated Lymphoid Tissue Formation through p38¿-Dependent Restraint of NF-¿B Signaling.
33-8700 was used in western blot to study epithelial control of gut-associated lymphoid tissue formation via p38alpha-dependent restraint of NF-kappaB signaling
|Caballero-Franco C,Guma M,Choo MK,Sano Y,Enzler T,Karin M,Mizoguchi A,Park JM||Journal of immunology (Baltimore, Md. : 1950) (196:2368)||2016|
|Mouse||Not Cited||p38¿ and p38¿ kinases regulate the Toll-like receptor 4 (TLR4)-induced cytokine production by controlling ERK1/2 protein kinase pathway activation.||Risco A,del Fresno C,Mambol A,Alsina-Beauchamp D,MacKenzie KF,Yang HT,Barber DF,Morcelle C,Arthur JS,Ley SC,Ardavin C,Cuenda A||Proceedings of the National Academy of Sciences of the United States of America (109:11200)||2012|
|Not Applicable||Not Cited||
Pomegranate extract inhibits the interleukin-1ß-induced activation of MKK-3, p38¿-MAPK and transcription factor RUNX-2 in human osteoarthritis chondrocytes.
33-8700 was used in western blot to study the effect of pomegranate extract on the IL-1beta-induced activation of MKK3/6, p38-MAPK, and RUNX-2 in primary human osteoarthritis chondrocytes
|Rasheed Z,Akhtar N,Haqqi TM||Arthritis research and therapy (12:null)||2010|
|Human||Not Cited||BIRB796 inhibits all p38 MAPK isoforms in vitro and in vivo.||Kuma Y,Sabio G,Bain J,Shpiro N,Márquez R,Cuenda A||The Journal of biological chemistry (280:19472)||2005|
Overexpression of mitogen-activated protein kinase kinase 6 in the heart improves functional recovery from ischemia in vitro and protects against myocardial infarction in vivo.
33-8700 was used in western blot to use transgenic mice to assess the effects of MKK6-mediated p38 activation in the heart.
|Martindale JJ,Wall JA,Martinez-Longoria DM,Aryal P,Rockman HA,Guo Y,Bolli R,Glembotski CC||The Journal of biological chemistry (280:669)||2005|
|Human||Not Cited||Basic fibroblast growth factor-induced cell death is effected through sustained activation of p38MAPK and up-regulation of the death receptor p75NTR.||Williamson AJ,Dibling BC,Boyne JR,Selby P,Burchill SA||The Journal of biological chemistry (279:47912)||2004|
|Rabbit||1:500||Stress kinase phosphorylation is increased in pacing-induced heart failure in rabbits.||Schulz R,Aker S,Belosjorow S,Konietzka I,Rauen U,Heusch G||American journal of physiology. Heart and circulatory physiology (285:H2084)||2003|
|Mouse||Not Cited||Cooperation of p38 and extracellular signal-regulated kinase mitogen-activated protein kinase pathways during granulocyte colony-stimulating factor-induced hemopoietic cell proliferation.||Rausch O,Marshall CJ||The Journal of biological chemistry (274:4096)||1999|
||p38¿ and p38¿ kinases regulate the Toll-like receptor 4 (TLR4)-induced cytokine production by controlling ERK1/2 protein kinase pathway activation.||Risco A,del Fresno C,Mambol A,Alsina-Beauchamp D,MacKenzie KF,Yang HT,Barber DF,Morcelle C,Arthur JS,Ley SC,Ardavin C,Cuenda A||Proceedings of the National Academy of Sciences of the United States of America (109:11200)||2012|
||Stress kinase phosphorylation is increased in pacing-induced heart failure in rabbits.||Schulz R,Aker S,Belosjorow S,Konietzka I,Rauen U,Heusch G||American journal of physiology. Heart and circulatory physiology (285:H2084)||2003|
Cyclosporine A induces nerve growth factor expression via activation of MAPK p38 and NFAT5.
33-8700 was used in western blot to study the effects of cyclosporine A on the mechanism of nerve growth factor expression using a corneal epithelial cell line.
|Lee JH,Kim JW,Im YS,Seong GJ,Lee HK||Cornea (30 Suppl 1:S19)||2011|