Western blot of p42 MAP Kinase/ERK2/MAPK1 in E. coli derived fusion protein using a p42 MAP Kinase/ERK2/MAPK1 polyclonal antibody (Product # PA1-20854) at a dilution of 1:2000 followed by detection using a Goat anti-IgY-HRP secondary antibody at a dilution of 1:1000 and Colorimetric signal development.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Recombinant fragment, corresponding to amino acids 232-360 of Human ERK2.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20854 detects ERK2 in Human, Mouse and Rat samples.
PA1-20854 has been successfully used in Western Blot procedures.
PA1-20854 immunogen corresponds to Recombinant fragment, corresponding to amino acids 232-360 of Human ERK2..
ERK2 is activated by MEK2, which phosphorylates neighbouring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.