|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide mapping within the subdomain XI of rat ERK1.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30387 detects ERK1 from rat, mouse, and human samples.
PA1-30387 has been successfully used in immunohistochemistry, immunoprecipitation, and Western blot applications.
The PA1-30387 immunogen is synthetic peptide mapping within the subdomain XI of rat ERK1.
MAP kinases consist of several subgroups, including the ERK, JNK, and p38 kinases. The members are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides. These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis. The MAPKs family is also characteried by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation. The closely related ERK1 (44kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif. While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is aslo activated by dual phosphorylation at a T-G-Y motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.