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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 350-400 of human ERK1.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: A-431 whole cell lysate1, HeLaS3, NIH-3T3, JC and BCL-1 cell lines, antigen standard for MAPK3 (transient overexpression lysate).
One of the major signaling pathways used by eukaryotic cells to translate extracellular signals into cellular responses is the MAP (mitogen-activated protein) kinase cascade. So far, four MAPK subgroups (ERK, ERK5, JNK and p38) have been identified in humans. ERK, extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ERK1; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; insulin-stimulated MAP2 kinase; MAP kinase isoform p44; MAPK 1; microtubule-associated protein 2 kinase
ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; MAPK3; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3