Immunofluorescent analysis of ERK1 (green) in untreated and siRNA knockdown treated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing ERK1 (Product # MA1-13041), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 549 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Full-length human recombinant protein expressed in bacteria.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 ul|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-13041 detects ERK1 from human, mouse, and monkey samples. MA1-13041 does not cross react with ERK2.
MA1-13041 has been successfully used in Western blot, ChIP, immunofluoresence, and immunohistochemistry procedures. By Western blot, this antibody detects a 44 kDa protein corresponding to ERK1.
The MA1-13041 antigen is a recombinant protein corresponding to the full-length sequence of human ERK1.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Engineering microdeletions and microduplications by targeting segmental duplications with CRISPR.
MA1-13041 was used in western blot to discuss the use of Crispr/Cas to develop models for non-allelic homologous recombination based diseases
|Tai DJ,Ragavendran A,Manavalan P,Stortchevoi A,Seabra CM,Erdin S,Collins RL,Blumenthal I,Chen X,Shen Y,Sahin M,Zhang C,Lee C,Gusella JF,Talkowski ME||Nature neuroscience (19:517)||2016|