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|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Full-length recombinant human p53|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is breast or colon carcinoma cells.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
IP-MS enrichment of TP53 (LFQ intensity): TP53 was enriched 3149-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and TP53 antibody (Product # MA1-12648). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Antigen NY-CO-13; Cellular tumor antigen p53; mutant p53; mutant tumor protein 53; p53 cellular tumor antigen; p53 tumor suppressor; Phosphoprotein p53; TP53; transformation related protein 53; transformation-related protein 53; tumor protein 53; tumor protein p53 (Li-Fraumeni syndrome); Tumor suppressor p53; tumor supressor p53
bbl; BCC7; bfy; bhy; LFS1; p44; P53; TP53; TRP53