Immunohistochemistry analysis of p53 showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p53 Mouse Monoclonal Antibody (MA512453) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Hamster, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||SV40-transformed mouse B4 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12453 targets p53 in FACS, IF, IP, and WB applications and shows reactivity with Hamster, Human, mouse, Non-human primate, and Rat samples.
The MA5-12453 immunogen is sV40-transformed mouse B4 cells.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
IP-MS enrichment of TP53 (LFQ intensity): TP53 was enriched 3083-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and TP53 antibody (Product # MA5-12453). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Simulated Microgravity Disrupts Cytoskeleton Organization and Increases Apoptosis of Rat Neural Crest Stem Cells Via Upregulating CXCR4 Expression and RhoA-ROCK1-p38 MAPK-p53 Signaling.
MA5-12453 was used in immunocytochemistry and western blot to elucidate an increase of apoptosis and disruption of cytoskeleton organization of rat neural crest stem cells via upregulating CXCR4 expression and RhoA-ROCK1-p38 MAPK-p53 signaling due to sim
|Lin SC,Gou GH,Hsia CW,Ho CW,Huang KL,Wu YF,Lee SY,Chen YH||Stem cells and development (25:1172)||2016|
p53 acts as a co-repressor to regulate keratin 14 expression during epidermal cell differentiation.
MA5-12453 was used in western blot to study keratin-14 downregulation during epidermal cell differentiation and the co-repressor role of p53
|Cai BH,Hsu PC,Hsin IL,Chao CF,Lu MH,Lin HC,Chiou SH,Tao PL,Chen JY||PloS one (7:null)||2012|
A novel p53-binding domain in CUL7.
MA5-12453 was used in western blot to study a novel p53-binding domain in CUL7
|Kasper JS,Arai T,DeCaprio JA||Biochemical and biophysical research communications (348:132)||2006|
Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53.
MA5-12453 was used in immunoprecipitation to study the role of p53 in the interaction between simian virus 40 large T antigen and human CBP/p300
|Borger DR,DeCaprio JA||Journal of virology (80:4292)||2006|
Gene expression and apoptosis induction in p53-heterozygous irradiated mice.
MA5-12453 was used in immunoprecipitation to study the expression of genes involved in cell cycle checkpoint activation and apoptosis in p53 heterozygous mice following X-ray irradiation
|di Masi A,Antoccia A,Dimauro I,Argentino-Storino A,Mosiello A,Mango R,Novelli G,Tanzarella C||Mutation research (594:49)||2006|
Retention of wild-type p53 in tumors from p53 heterozygous mice: reduction of p53 dosage can promote cancer formation.
MA5-12453 was used in immunoprecipitation to study whether the loss of both p53 tumor suppressor alleles is required for tumor progression
|Venkatachalam S,Shi YP,Jones SN,Vogel H,Bradley A,Pinkel D,Donehower LA||The EMBO journal (17:4657)||1998|
p53 associates with and targets Delta Np63 into a protein degradation pathway.
MA5-12453 was used in immunocytochemistry and immunoprecipitation to study the mechanism by which p53 targets Delta Np63 into a protein degradation pathway
|Ratovitski EA,Patturajan M,Hibi K,Trink B,Yamaguchi K,Sidransky D||Proceedings of the National Academy of Sciences of the United States of America (98:1817)||2001|