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Immunofluorescence analysis of p53 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled p53 (PAb 240) Mouse Monoclonal Antibody (MA515244) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Rhesus monkey|
|Published species reactivity||Rabbit, Rat, Cat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Murine p53 protein corresponding to aa14-289|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 µg x 10^6 cells|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 16 publications below|
|Immunohistochemistry (IHC)||See 24 publications below|
|Immunoprecipitation (IP)||See 6 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
MA5-15244 targets p53 in FACS, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-15244 immunogen is mouse p53 protein corresponding to aa14-289.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
IP-MS enrichment of TP53 (LFQ intensity): TP53 was enriched 1135-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and TP53 antibody (Product # MA5-15244). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IFI27, a novel epidermal growth factor-stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes.
MA5-15244 was used in western blot to identify the role of IFI27 in the cell proliferation of human epidermal keratinocytes
|Hsieh WL,Huang YH,Wang TM,Ming YC,Tsai CN,Pang JH||Cell proliferation (48:187)||2015|
Two mechanisms underlying the loss of p16(Ink4a) function are associated with distinct tumorigenic consequences for WS MEFs escaping from senescence.
MA5-15244 was used in western blot to study the tumorigenic consequences of different mechanisms of p16(Ink4a) loss of function
|Wu X,Jia S,Zhang X,Si X,Tang W,Luo Y||Mechanisms of ageing and development (133:549)||2012|
Pu-erh tea inhibits tumor cell growth by down-regulating mutant p53.
MA5-15244 was used in western blot to investigate the effect of Pu-erh tea on cancer cell growth and its mechanism
|Zhao L,Jia S,Tang W,Sheng J,Luo Y||International journal of molecular sciences (12:7581)||2011|
Exploring antibody recognition of sequence space through random-sequence peptide microarrays.
MA5-15244 was used in western blot to study the feasibility of using a random peptide microarray to map antibody epitopes
|Halperin RF,Stafford P,Johnston SA||Molecular & cellular proteomics : MCP (10:null)||2011|
In silico analysis of kinase expression identifies WEE1 as a gatekeeper against mitotic catastrophe in glioblastoma.
MA5-15244 was used in western blot to perform bioinformatic analyses of kinase expression to determine the role of WEE1 in glioblastoma
|Mir SE,De Witt Hamer PC,Krawczyk PM,Balaj L,Claes A,Niers JM,Van Tilborg AA,Zwinderman AH,Geerts D,Kaspers GJ,Peter Vandertop W,Cloos J,Tannous BA,Wesseling P,Aten JA,Noske DP,Van Noorden CJ,Würdinger T||Cancer cell (18:244)||2010|
Cyclin A is essential for the p53-modulated inhibition from benzo(a)pyrene toxicity in A549 cells.
MA5-15244 was used in western blot to study the role of cyclin A in the protective effect of p53 from benzopyrene carcinogenic toxicity in A549 cells
|Wang Z,Qi Y,Chen Q,Yang D,Tang S,Jin X,Gao J,Fu J,Zhou Z,Wang J,Xiao X||Toxicology (256:1)||2009|
Ku80 deletion suppresses spontaneous tumors and induces a p53-mediated DNA damage response.
MA5-15244 was used in western blot to investigate the effect of Ku80 on tumor growth
|Holcomb VB,Rodier F,Choi Y,Busuttil RA,Vogel H,Vijg J,Campisi J,Hasty P||Cancer research (68:9497)||2008|
Histone deacetylase inhibitor trichostatin A induced caspase-independent apoptosis in human gastric cancer cell.
MA5-15244 was used in western blot to study the mechanism by which a histone deacetylase inhibitor induces apoptosis in human gastric cancer cells
|Wu ZQ,Zhang R,Chao C,Zhang JF,Zhang YQ||Chinese medical journal (120:2112)||2007|
Altered gene expression in cells from patients with lysosomal storage disorders suggests impairment of the ubiquitin pathway.
MA5-15244 was used in western blot to study the involvement of the ubiquitin pathway in lysosomal storage disorders
|Bifsha P,Landry K,Ashmarina L,Durand S,Seyrantepe V,Trudel S,Quiniou C,Chemtob S,Xu Y,Gravel RA,Sladek R,Pshezhetsky AV||Cell death and differentiation (14:511)||2007|
Elevated telomere-telomere recombination in WRN-deficient, telomere dysfunctional cells promotes escape from senescence and engagement of the ALT pathway.
MA5-15244 was used in western blot to study the role elevated telomere-telomere recombination and escape from senescence in the pathogenesis of Werner Syndrome
|Laud PR,Multani AS,Bailey SM,Wu L,Ma J,Kingsley C,Lebel M,Pathak S,DePinho RA,Chang S||Genes & development (19:2560)||2005|
p53 targets simian virus 40 large T antigen for acetylation by CBP.
MA5-15244 was used in western blot to study the role of p53 in targeting simian virus 40 large T antigen for acetylation by CBP
|Poulin DL,Kung AL,DeCaprio JA||Journal of virology (78:8245)||2004|
Cul7/p185/p193 binding to simian virus 40 large T antigen has a role in cellular transformation.
MA5-15244 was used in western blot to study the role of p185/Cul7 binding to simian virus 40 large T antigen in cellular transformation
|Ali SH,Kasper JS,Arai T,DeCaprio JA||Journal of virology (78:2749)||2004|
Expression of angiotensin type II receptor downregulates Cdk4 synthesis and inhibits cell-cycle progression.
MA5-15244 was used in western blot to study the role of angiotensin II receptor expression in Cdk4 downregulation and inhibition of cell proliferation
|Gingras B,Rodier G,Giasson E,Coulombe P,Chassagne C,Meloche S||Oncogene (22:2633)||2003|
Changes in expression and immunolocalization of protein associated with toxic bile salts-induced apoptosis in rat hepatocytes.
MA5-15244 was used in western blot to study the molecular mechanism of toxic bile salts-induced apoptosis in rat hepatocytes
|Oh SH,Yun KJ,Nan JX,Sohn DH,Lee BH||Archives of toxicology (77:110)||2003|
Selective impairment of p53-mediated cell death in fibroblasts from sporadic Alzheimer's disease patients.
MA5-15244 was used in western blot to study the selective impairment of p53-mediated cell death in fibroblasts from sporadic Alzheimer's disease patients
|Uberti D,Carsana T,Bernardi E,Rodella L,Grigolato P,Lanni C,Racchi M,Govoni S,Memo M||Journal of cell science (115:3131)||2002|
The human MDM2 oncoprotein increases the transcriptional activity and the protein level of the p53 homolog p63.
MA5-15244 was used in western blot to investigate the effect of MDM2 oncoprotein on the expression of p63
|Calabrò V,Mansueto G,Parisi T,Vivo M,Calogero RA,La Mantia G||The Journal of biological chemistry (277:2674)||2002|
Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.
MA5-15244 was used in immunohistochemistry to study apoptosis induced by p53 in response to DNA damage and the essential role of c-Myc
|Phesse TJ,Myant KB,Cole AM,Ridgway RA,Pearson H,Muncan V,van den Brink GR,Vousden KH,Sears R,Vassilev LT,Clarke AR,Sansom OJ||Cell death and differentiation (21:956)||2014|
Molecular profiling of parathyroid hyperplasia, adenoma and carcinoma.
MA5-15244 was used in immunohistochemistry to study the molecular profiles of parathyroid hyperplasia, adenoma and carcinoma
|Árvai K,Nagy K,Barti-Juhász H,Peták I,Krenács T,Micsik T,Végső G,Perner F,Szende B||Pathology oncology research : POR (18:607)||2012|
UV-induced ablation of the epidermal basal layer including p53-mutant clones resets UV carcinogenesis showing squamous cell carcinomas to originate from interfollicular epidermis.
MA5-15244 was used in immunohistochemistry to investigate the origin of UV-induced squamous cell carcinomas.
|Rebel HG,Bodmann CA,van de Glind GC,de Gruijl FR||Carcinogenesis (33:714)||2012|
Diagnostic value of progesterone receptor and p53 expression in uterine smooth muscle tumors.
MA5-15244 was used in immunohistochemistry to study the diagnostic value of progesterone receptor and p53 expression in uterine smooth muscle tumors
|Hewedi IH,Radwan NA,Shash LS||Diagnostic pathology (7:null)||2012|
Topical grape seed proanthocyandin extract reduces sunburn cells and mutant p53 positive epidermal cell formation, and prevents depletion of Langerhans cells in an acute sunburn model.
MA5-15244 was used in immunohistochemistry to study the protective effects of topical grape seed proanthocyandin extract in an acute sunburn model
|Yuan XY,Liu W,Hao JC,Gu WJ,Zhao YS||Photomedicine and laser surgery (30:20)||2012|
CIS is a surrogate marker of genetic instability and field carcinogenesis in the urothelial mucosa.
MA5-15244 was used in immunohistochemistry to study the value of CIS as a surrogate marker of genetic instability and field carcinogenesis in the urothelial mucosa
|Palmeira C,Lameiras C,Amaro T,Lima L,Koch A,Lopes C,Oliveira PA,Santos L||Urologic oncology (29:205)||2011|
Prognostic significance of mucin and p53 expression in stage IB non-small cell lung cancer: a laboratory companion study to CALGB 9633.
MA5-15244 was used in immunohistochemistry to evaluate mucin and p53 expression as prognostic markers for stage IB non-small cell lung cancer
|Graziano SL,Gu L,Wang X,Tatum AH,Vollmer RT,Strauss GM,Kratzke R,Dudek AZ,Vokes EE,Green MR||Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer (5:810)||2010|
ITF-2 is disrupted via allelic loss of chromosome 18q21, and ITF-2B expression is lost at the adenoma-carcinoma transition.
MA5-15244 was used in immunohistochemistry to study the expression and role of ITF-2B in colorectal carcinogenesis
|Herbst A,Bommer GT,Kriegl L,Jung A,Behrens A,Csanadi E,Gerhard M,Bolz C,Riesenberg R,Zimmermann W,Dietmaier W,Wolf I,Brabletz T,Göke B,Kolligs FT||Gastroenterology (137:639)||2009|
A limited role for p53 in modulating the immediate phenotype of Apc loss in the intestine.
MA5-15244 was used in immunohistochemistry to study the role of p53 in modulating the immediate phenotype of Apc loss in the intestine
|Reed KR,Meniel VS,Marsh V,Cole A,Sansom OJ,Clarke AR||BMC cancer (8:null)||2008|
Survivin, p53, and Ki-67 as predictors of histopathologic response in locally advanced rectal cancer treated with preoperative chemoradiotherapy.
MA5-15244 was used in immunohistochemistry to study the value of survivin, p53, and Ki-67 in predicting the response to preoperative chemoradiotherapy in locally advanced rectal cancer
|Terzi C,Canda AE,Sagol O,Atila K,Sonmez D,Fuzun M,Gorken IB,Oztop I,Obuz F||International journal of colorectal disease (23:37)||2008|
Growth stimulation of UV-induced DNA damage retaining epidermal basal cells gives rise to clusters of p53 overexpressing cells.
MA5-15244 was used in immunohistochemistry to investigate the mechanism for the pathogenesis of UV-induced melanomas
|Nijhof JG,Mulder AM,Speksnijder EN,Hoogervorst EM,Mullenders LH,de Gruijl FR||DNA repair (6:1642)||2007|
53BP1 deficiency in intestinal enterocytes does not alter the immediate response to ionizing radiation, but leads to increased nuclear area consistent with polyploidy.
MA5-15244 was used in immunohistochemistry to study the role of 53BP1 in mediating apoptosis and proliferation in mouse small intestine following gamma-irradiation
|Clarke AR,Jones N,Pryde F,Adachi Y,Sansom OJ||Oncogene (26:6349)||2007|
P53 expression between 13-27 weeks old human male fetus gonads.
MA5-15244 was used in immunohistochemistry to study the time-course of p53 expression in human male fetal gonads
|Tosun M,Tosun E,Kalkan S,Avunduk MC||Journal of molecular histology (38:271)||2007|
Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays.
MA5-15244 was used in immunohistochemistry to experimentally assess a mathematical model of the p53-Mdm2 feedback loop
|Ramalingam S,Honkanen P,Young L,Shimura T,Austin J,Steeg PS,Nishizuka S||Cancer research (67:6247)||2007|
Diabetes alters expression of p53 and c-myc in different stages of oral oncogenesis.
MA5-15244 was used in immunohistochemistry to study the expression of p53 and c-myc by normal and diabetic rats in a model of oral carcinogenesis
|Vairaktaris E,Kalokerinos G,Goutzanis L,Spyridonidou S,Vassiliou S,Derka S,Nkenke E,Yapijakis C,Vylliotis A,Lazaris A,Patsouris E||Anticancer research (27:1465)||2007|
Effects of extracellular matrix on the morphology and behaviour of rabbit auricular chondrocytes in culture.
MA5-15244 was used in immunohistochemistry to investigate cell apoptosis of cultured rabbit auricular chondrocytes under different circumstances
|Villar-Suárez V,Colaço B,Calles-Venal I,Bravo IG,Fernández-Alvarez JG,Fernández-Caso M,Villar-Lacilla JM||Journal of biomedicine & biotechnology (2005:364)||2006|
Mutations in Apc and p53 synergize to promote mammary neoplasia.
MA5-15244 was used in immunohistochemistry to study the synergistic role of Apc and p53 mutations in promoting mammary neoplasia
|Méniel V,Hay T,Douglas-Jones A,Sansom OJ,Clarke AR||Cancer research (65:410)||2005|
Immunohistochemical studies concerning the neuronal cell cycle of the cat using PCNA, Ki-67 and p53 markers.
MA5-15244 was used in immunohistochemistry to study the immunohistochemistry of markers of the cat neuronal cell cycle
|Gruber A,Schmidt P,Url A||Journal of veterinary medicine. A, Physiology, pathology, clinical medicine (51:416)||2004|
p53 heterozygosity results in an increased 2-acetylaminofluorene-induced urinary bladder but not liver tumor response in DNA repair-deficient Xpa mice.
MA5-15244 was used in immunohistochemistry to study the role of p53 heterozygosity in increased 2-acetylaminofuorene-induced cancer in DNA repair-deficient Xpa mice
|Hoogervorst EM,van Oostrom CT,Beems RB,van Benthem J,Gielis S,Vermeulen JP,Wester PW,Vos JG,de Vries A,van Steeg H||Cancer research (64:5118)||2004|
p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma.
MA5-15244 was used in immunohistochemistry to study the molecular determinants of the response to radiotherapy in oral carcinoma
|Jayasurya R,Francis G,Kannan S,Lekshminarayanan K,Nalinakumari KR,Abraham T,Abraham EK,Nair MK||International journal of cancer (109:710)||2004|
Oral cytology assessment by flow cytometry of DNA adducts, aneuploidy, proliferation and apoptosis shows differences between smokers and non-smokers.
MA5-15244 was used in immunohistochemistry to study the differences in oral cytology between smokers and non-smokers
|Schwartz JL,Muscat JE,Baker V,Larios E,Stephenson GD,Guo W,Xie T,Gu X,Chung FL||Oral oncology (39:842)||2003|
Synergistic decline in expressions of p73 and p21 with invasion in esophageal cancers.
MA5-15244 was used in immunohistochemistry to investigate the correlation between changes of p73 and p21 level and esophageal cancer status
|Masuda N,Kato H,Nakajima T,Sano T,Kashiwabara K,Oyama T,Kuwano H||Cancer science (94:612)||2003|
The predictive value of neuroendocrine markers and p53 for response to chemotherapy and survival in patients with advanced non-small cell lung cancer.
MA5-15244 was used in immunohistochemistry to study the value of neuroendocrine markers and p53 for predicting chemotherapeutic response and survival in advanced non-small cell lung cancer
|Gajra A,Tatum AH,Newman N,Gamble GP,Lichtenstein S,Rooney MT,Graziano SL||Lung cancer (Amsterdam, Netherlands) (36:159)||2002|
Use of neuroendocrine markers, p53, and HER2 to predict response to chemotherapy in patients with stage III non-small cell lung cancer: a Cancer and Leukemia Group B study.
MA5-15244 was used in immunohistochemistry to study whether neuroendocrine markers, p53, and HER2 can predict response to chemotherapy in patients with stage III non-small cell lung cancer
|Graziano SL,Tatum A,Herndon JE,Box J,Memoli V,Green MR,Kern JA||Lung cancer (Amsterdam, Netherlands) (33:115)||2001|
Zyxin is a novel target for β-amyloid peptide: characterization of its role in Alzheimer's pathogenesis.
MA5-15244 was used in immunoprecipitation to study the ability of beta-amyloid (1-40) and beta-amyloid (1-42) to interfere with the expression of zyxin
|Lanni C,Necchi D,Pinto A,Buoso E,Buizza L,Memo M,Uberti D,Govoni S,Racchi M||Journal of neurochemistry (125:790)||2013|
Conformational altered p53 affects neuronal function: relevance for the response to toxic insult and growth-associated protein 43 expression.
MA5-15244 was used in immunoprecipitation to study the role of p53 with an altered conformation in reduced GAP-43 expression, impaired neuronal function and reduced apoptosis
|Buizza L,Prandelli C,Bonini SA,Delbarba A,Cenini G,Lanni C,Buoso E,Racchi M,Govoni S,Memo M,Uberti D||Cell death & disease (4:null)||2013|
Wild type but not mutant APP is involved in protective adaptive responses against oxidants.
MA5-15244 was used in immunoprecipitation to investigate the role of APP in protective adaptive responses agaist oxidants
|Cenini G,Maccarinelli G,Lanni C,Bonini SA,Ferrari-Toninelli G,Govoni S,Racchi M,Butterfield DA,Memo M,Uberti D||Amino acids (39:271)||2010|
The ubiquitin-proteasome system regulates p53-mediated transcription at p21waf1 promoter.
MA5-15244 was used in immunoprecipitation and western blot to study the role of the ubiquitin-proteasome system in the transcriptional activation function of p53
|Zhu Q,Wani G,Yao J,Patnaik S,Wang QE,El-Mahdy MA,Praetorius-Ibba M,Wani AA||Oncogene (26:4199)||2007|
Nerve growth factor restores p53 function in pituitary tumor cell lines via trkA-mediated activation of phosphatidylinositol 3-kinase.
MA5-15244 was used in immunoprecipitation to study the role of trkA and PI3kinase in the restorationof p53 function by NGF in pituitary tumor cell lines
|Facchetti M,Uberti D,Memo M,Missale C||Molecular endocrinology (Baltimore, Md.) (18:162)||2004|
Transformation of bone marrow B-cell progenitors by E2a-Hlf requires coexpression of Bcl-2.
MA5-15244 was used in immunoprecipitation to study the role of BCL-2 in the transformation of bone marrow B-cell progenitors by E2a-Hlf
|Smith KS,Rhee JW,Cleary ML||Molecular and cellular biology (22:7678)||2002|
Searching for predictive blood biomarkers: misfolded p53 in mild cognitive impairment.
MA5-15244 was used in flow cytometry to study the ability of cytofluorometric measurement of misfolded p53 in venous blood samples to predict progression from mild cognitive disorder to Alzheimer's disease
|Stanga S,Lanni C,Sinforiani E,Mazzini G,Racchi M||Current Alzheimer research (9:1191)||2012|
Unfolded p53 in blood as a predictive signature signature of the transition from mild cognitive impairment to Alzheimer's disease.
MA5-15244 was used in flow cytometry to study the predictive value of unfolded p53 in blood for the development of Alzheimer's disease
|Lanni C,Racchi M,Stanga S,Mazzini G,Ranzenigo A,Polotti R,Memo M,Govoni S,Uberti D||Journal of Alzheimer's disease : JAD (20:97)||2010|
The gain of function of p53 mutant p53S in promoting tumorigenesis by cross-talking with H-RasV12.
MA5-15244 was used in ChIP assay to study the role of H-RasV12 in the promotion of tumorigenesis by a gain of function p53 mutant
|Jia S,Zhao L,Tang W,Luo Y||International journal of biological sciences (8:596)||2012|
Multiple initial culture conditions enhance the establishment of cell lines from primary ovarian cancer specimens.
MA5-15244 was used in immunocytochemistry to establish stable ovarian tumor cell lines
|Bertozzi CC,Chang CY,Jairaj S,Shan X,Huang J,Weber BL,Chu CS,Carroll RG||In vitro cellular & developmental biology. Animal (42:58)||2006|
Cell death induced by taxanes in breast cancer cells: cytochrome C is released in resistant but not in sensitive cells.
MA5-15244 was used in immunocytochemistry to study the mechanisms responsible for the resistance of breast cancer cells to taxanes
|Ehrlichová M,Koc M,Truksa J,Naldová Z,Václavíková R,Kovárr J||Anticancer research (25:4215)||2005|
antigen NY-CO-13; cellular tumor antigen p53; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; TP53; transformation-related protein 53; tumor protein 53; tumor suppressor p53; tumor supressor p53
BCC7; EGK_08142; LFS1; P53; TP53; TRP53