|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human full length wild type p53 protein.|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Quantitative apparent diffusion coefficient measurements obtained by 3-Tesla MRI are correlated with biomarkers of bladder cancer proliferative activity.
MA5-16387 was used in immunohistochemistry to study 3-Tesla MRI diffusion coefficient measurements correlated with biomarkers of bladder cancer proliferative activity
|Sevcenco S,Haitel A,Ponhold L,Susani M,Fajkovic H,Shariat SF,Hiess M,Spick C,Szarvas T,Baltzer PA||PloS one (9:null)||2014|
Tissue biomarkers in prognostication of serous ovarian cancer following neoadjuvant chemotherapy.
MA5-16387 was used in immunohistochemistry to attempt to identify prognostic biomarkers in serous ovarian cancer patients treated with neoadjuvant chemotherapy
|Khandakar B,Mathur SR,Kumar L,Kumar S,Datta Gupta S,Iyer VK,Kalaivani M||BioMed research international (2014:null)||2014|
Quantitative analysis of ¿-H2AX and p53 nuclear expression levels in ovarian and fallopian tube epithelium from risk-reducing salpingo-oophorectomies in BRCA1 and BRCA2 mutation carriers.
MA5-16387 was used in immunohistochemistry to study ovarian and fallopian tube epithelial expression of gamma-H2AX and p53 in patients with BRCA1 and BRCA2 mutations undergoing salpingo-oophrectomy
|Staff S,Tolonen T,Laasanen SL,Mecklin JP,Isola J,Mäenpää J||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (33:309)||2014|
Genetic characterization of mesenchymal, clear cell, and dedifferentiated chondrosarcoma.
MA5-16387 was used in immunohistochemistry to study the genetics of mesenchymal, clear cell and dedifferentiated chondrosarcoma and the potential involvement of the retinoblastoma pathway
|Meijer D,de Jong D,Pansuriya TC,van den Akker BE,Picci P,Szuhai K,Bovée JV||Genes, chromosomes and cancer (51:899)||2012|
Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors.
MA5-16387 was used in immunohistochemistry to study the diagnostic value of the immunohistochemical expression of apoptotic and anti-apoptotic markers in serous and mucinous ovarian tumors
|Ozer H,Yenicesu G,Arici S,Cetin M,Tuncer E,Cetin A||Diagnostic pathology (7:null)||2012|
Proliferation and maturation of microvessels in arteriovenous malformations--expression patterns of angiogenic and cell cycle-dependent factors.
MA5-16387 was used in immunohistochemistry to study the patterns of proliferation and maturation of microvessels in arteriovenous malformations
|Meijer-Jorna LB,van der Loos CM,Teeling P,de Boer OJ,Florquin S,van der Horst CM,van der Wal AC||Journal of cutaneous pathology (39:610)||2012|
Evaluation of immunohistochemical expression of p53, p21, p27, cyclin D1, and Ki67 in oral and oropharyngeal squamous cell carcinoma.
MA5-16387 was used in immunohistochemistry to study the immunohistochemical expression of p53, p21, p27, cyclin D1 and Ki67 in oral and oropharyngeal squamous cell carcinoma
|Perisanidis C,Perisanidis B,Wrba F,Brandstetter A,El Gazzar S,Papadogeorgakis N,Seemann R,Ewers R,Kyzas PA,Filipits M||Journal of oral pathology and medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (41:40)||2012|
Recurrence of benign meningiomas: predictive value of proliferative index, BCL2, p53, hormonal receptors and HER2 expression.
MA5-16387 was used in immunohistochemistry to identify biomarkers that predict the recurrence of benign meningiomas
|Abdelzaher E,El-Gendi SM,Yehya A,Gowil AG||British journal of neurosurgery (25:707)||2011|
Osteosarcoma arising in a long-standing uterine leiomyoma: a case report and literature review.
MA5-16387 was used in immunohistochemistry to report on a case of osteosarcoma occurring in a long-standing uterine leiomyoma
|Wang RC,Wen MC,Wang J,Ho SC,Jan YJ||International journal of surgical pathology (19:99)||2011|
Extent and patterns of MGMT promoter methylation in glioblastoma- and respective glioblastoma-derived spheres.
MA5-16387 was used in immunohistochemistry to study MGMT promoter methylation in glioblastoma- and respective glioblastoma-derived spheres
|Sciuscio D,Diserens AC,van Dommelen K,Martinet D,Jones G,Janzer RC,Pollo C,Hamou MF,Kaina B,Stupp R,Levivier M,Hegi ME||Clinical cancer research : an official journal of the American Association for Cancer Research (17:255)||2011|
Correlation of immunohistochemical expression of p53 with unamplified chromosome 17 polysomy in invasive breast carcinoma.
MA5-16387 was used in immunohistochemistry to study the correlation of p53 expression with unamplified chromosome 17 polysomy in invasive breast carcinoma
|Krishnamurti U,Zarineh A,Atem FD,Silverman JF||Applied immunohistochemistry and molecular morphology : AIMM (19:28)||2011|
Expression of senescence-related genes in human corneal endothelial cells.
MA5-16387 was used in immunohistochemistry to study the expression of senescence-related genes in human corneal endothelial cells from donors of different ages
|Song Z,Wang Y,Xie L,Zang X,Yin H||Molecular vision (14:161)||2008|
|Not Applicable||Not Cited||
Evaluation of correlation of cell cycle proteins and Ki-67 interaction in paranasal sinus inverted papilloma prognosis and squamous cell carcinoma transformation.
MA5-16387 was used in immunohistochemistry - paraffin section to study the correlation of Ki-67 and cell cycle proteins in paranasal sinus inverted papilloma prognosis and squamous cell carcinoma transformation
|Tsou YA,Huang HJ,Wang TC,Tai CJ,Chen CM,Chen CY||BioMed research international (2014:null)||2014|