|ChIP assay (ChIP)||1-3 µg x 10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||Assay-Dependent|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Tested Species reactivity||Human, Rhesus monkey|
|Published species reactivity||Rat , Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human wild type p53 protein expressed in E. coli.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
This antibody reacts with mutant and wild form p53. It reacts with an epitope at aa 20-25.
Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min.
Recommended positive controls include SK-BR-3 cells, MDA-231 cells, and colon carcinoma.
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; mutant tumor protein 53; P53; p53 tumor suppressor; Phosphoprotein p53; TP53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53
Gene Aliases: BCC7; EGK_08142; LFS1; P53; TP53; TRP53
Molecular Function: transcription factor
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.