Immunohistochemistry analysis of p57Kip2 showing staining in the nucleus of paraffin-embedded mouse placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p57Kip2 Rabbit Polyclonal Antibody (PA516539) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Human, Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from human p57Kip2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16539 targets p57Kip2 in IHC (P) applications and shows reactivity with mouse, Bovine, and Human samples.
The PA5-16539 immunogen is a synthetic peptide from human p57Kip2 protein.
p57Kip2 (or CDKN1C) is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57Kip2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith-Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF-II) and H19, both of which seem to be implicated in adrenal neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming.
PA5-16539 was used in western blot to study modification of intrauterine programming by a decrease in amino acid and glucose transporters and an increase in rat placental fatty acid
|Nüsken E,Gellhaus A,Kühnel E,Swoboda I,Wohlfarth M,Vohlen C,Schneider H,Dötsch J,Nüsken KD||Journal of cellular biochemistry (117:1594)||2016|
Transcriptional regulatory mechanisms underlying the GABAergic neuron fate in different diencephalic prosomeres.
PA5-16539 was used in immunohistochemistry to study the fate of GABAergic neurons in different diencephalic prosomeres and the transcriptional mechanisms involved
|Virolainen SM,Achim K,Peltopuro P,Salminen M,Partanen J||Development (Cambridge, England) (139:3795)||2012|