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Immunohistochemistry analysis of p57Kip2 showing staining in the nucleus of paraffin-embedded mouse placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p57Kip2 Rabbit Polyclonal Antibody (PA516539) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from human p57Kip2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-16539 targets p57Kip2 in IHC (P) applications and shows reactivity with mouse, Bovine, and Human samples.
The PA5-16539 immunogen is a synthetic peptide from human p57Kip2 protein.
p57Kip2 (or CDKN1C) is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57Kip2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith-Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF-II) and H19, both of which seem to be implicated in adrenal neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transcriptional regulatory mechanisms underlying the GABAergic neuron fate in different diencephalic prosomeres.
PA5-16539 was used in immunohistochemistry to study the fate of GABAergic neurons in different diencephalic prosomeres and the transcriptional mechanisms involved
|Virolainen SM,Achim K,Peltopuro P,Salminen M,Partanen J||Development (Cambridge, England) (139:3795)||2012|
cyclin-dependent kinase inhibitor 1C; cyclin-dependent kinase inhibitor 1C (p57, Kip2); cyclin-dependent kinase inhibitor p57
AL024410; BOS_25086; BWCR; BWS; CDKI; CDKN1C; KIP2; p57; p57(kip2); p57Kip2; WBS