|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Full length protein (Human)|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23147 detects p73 in Human and Mouse samples. Does not cross-reat with p53.
MA1-23147 has been successfully used in Immunofluorescence, Immunoprecipitation and Western Blot procedures.
MA1-23147 immunogen corresponds to Full length protein (Human).
p73 protein is a member of the p53 family of proteins. The tumor-suppressor protein p53 exhibits sequence specific DNA-binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage. In response to signals generated by a variety of genotoxic stresses, e.g, UV irradiation or DNA damage, p53 is expressed and undergoes post-translational modification that results in its accumulation in the nucleus. Activation of p53 leads to cell cycle arrest and in some cases to apoptosis, resulting in the inability of genetically damaged cells to proliferate. Thus, the p53-dependent pathways help to maintain genomic stability by eliminating damaged cells. The accumulation of high levels of p53 is a potential marker for malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.