Western blot analysis of p84 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate (D) 30 µg HepG2 whole cell lysate and E) 30 µg A375 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a p84 monoclonal antibody (Product # MA1-23261) at a dilution of 1:500.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Amino acids 15-374 of human p84 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Immunocytochemistry (ICC)||0.5 - 2 ug/ml|
|Immunofluorescence (IF)||0.5-2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||0.3-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23261 detects p84 (MS - P8410 - PX1) in Human, mouse, rat and monkey samples. Suggested positive controls are HeLa, Raji, and MOLT4.
MA1-23261 has been successfully used in Immunofluorescence, Immunoprecipitation Immunohistochemistry, ChIP, and Western Blot procedures.
MA1-23261 immunogen corresponds to Amino acids 15-374 of human p84 expressed in E. coli..
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.