Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Hippocampus tissue using PA5-19479, anti-Pan Cadherin antibody. Primary antibody was used at a concentration of 1 ug/ml and exposed for 8 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human pan Cadherin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
A diffuse signal may be seen at higher concentrations (5-10 µg/ml) in ICC/IF.
For Western Blot, this antibody has non-specific bands at 40 kDa,65 kDa,75 kDa and 90 kDa.
This antibody is predicted to react with chicken, cow and Xenopus laevis based on sequence homology.
This antibody may not perform well in ICC/IF using mouse cells.
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images.
PA5-19479 was used in immunohistochemistry to discuss confocal microscopy imaging of intact tissues
|Chiang M,Hallman S,Cinquin A,de Mochel NR,Paz A,Kawauchi S,Calof AL,Cho KW,Fowlkes CC,Cinquin O||BMC bioinformatics (16:null)||2015|