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|Tested species reactivity||Human|
|Published species reactivity||Rat, Fish, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeleton preparation from human A431 carcinoma Cells.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects cytokeratins 4, 5, 6, 8, 10, 13, 18.
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the
molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
Reacting with a variety of keratins (4,5,6,8,10,13 and 18) which reacts with a variety of normal reactive and neoplasmic epithelia. Reacting with simple epithelium and both basal and superbasal layers of comfying and non comfying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Optimisation of immunofluorescence methods to determine MCT1 and MCT4 expression in circulating tumour cells.
MA1-12594 was used in western blot to develop an immunofluorescence method to assess MCT1 and MCT4 in circulating tumor cells and as them as biomarkers of AZD3965 in cancer patients
|Kershaw S,Cummings J,Morris K,Tugwood J,Dive C||BMC cancer (15:null)||2015|
Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells.
MA1-12594 was used in western blot to study the role of keratins in modulating cFlip and ERK1/2 apoptotic signaling in epithelial cells
|Gilbert S,Loranger A,Marceau N||Molecular and cellular biology (24:7072)||2004|
Characterization of goldfish fin cells in culture: some evidence of an epithelial cell profile.
MA1-12594 was used in immunocytochemistry to characterize goldfish fin cells through morphological, immunochemical and molecular methods
|Mauger PE,Labbé C,Bobe J,Cauty C,Leguen I,Baffet G,Le Bail PY||Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology (152:205)||2009|
Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development.
MA1-12594 was used in immunohistochemistry to study the essential role of mind bomb 1 in the development of T cells and marginal zone B cells
|Song R,Kim YW,Koo BK,Jeong HW,Yoon MJ,Yoon KJ,Jun DJ,Im SK,Shin J,Kong MP,Kim KT,Yoon K,Kong YY||The Journal of experimental medicine (205:2525)||2008|
Inhibition of VEGFR2 prevents DMBA-induced mammary tumor formation.
MA1-12594 was used in immunohistochemistry to investigate the effect of VEGFR2 blockade on breast cancer development stimulated by DMBA
|Heffelfinger SC,Yan M,Gear RB,Schneider J,LaDow K,Warshawsky D||Laboratory investigation; a journal of technical methods and pathology (84:989)||2004|
TNP-470 inhibits 7,12-dimethylbenz[a]anthracene-induced mammary tumor formation when administered before the formation of carcinoma in situ but is not additive with tamoxifen.
MA1-12594 was used in immunohistochemistry to investigate the effect of TNP-470 on breast tumor development stimulated with DMBA
|Heffelfinger SC,Gear RB,Schneider J,LaDow K,Yan M,Lu F,Pyle AL,Warshawsky D||Laboratory investigation; a journal of technical methods and pathology (83:1001)||2003|
Evidence for modulation of a 24K protein in human endometrium during the menstrual cycle.
MA1-12594 was used in immunohistochemistry to investigate the expression and localization of 24K protein in the endometrium of regularly cycling women
|Ciocca DR,Asch RH,Adams DJ,McGuire WL||The Journal of clinical endocrinology and metabolism (57:496)||1983|
58 kda cytokeratin; cell proliferation-inducing gene 46 protein; CK-10; CK-13; CK-5; cytokeratin 10; cytokeratin 13; cytokeratin 18; cytokeratin 4; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; cytokeratin-13; cytokeratin-5; cytokeratin-6C; cytokeratin-6E; cytokeratin-8; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 10, type I; keratin 13, type I; keratin 18, type I; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 8, type II; keratin K6h; keratin, epidermal type II, K6A; keratin, type II cytoskeletal 6A; keratin-10; keratin-13; keratin-6C; pankeratin; type-II keratin Kb12; type-II keratin Kb4; type-II keratin Kb5; type-II keratin Kb6; type-II keratin Kb8
BCIE; BIE; CARD2; CK-18; CK-4; CK-6C; CK-6E; CK-8; CK10; CK13; CK4; CK5; CK6A; CK6C; CK6D; CK8; CYK18; CYK4; CYK8; DDD; DDD1; EBS2; EHK; K10; K13; K18; K2C8; K4; K5; K6A; K6C; K6D; K8; KO; KPP; KRT10; KRT13; KRT18; KRT5; KRT5A; KRT6A; KRT6C; KRT6D; KRT8; PC3; PIG46; WSN1; WSN2