|Tested species reactivity||Human, Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||A synthetic peptide (AKEGVVAAAEKTKQGV) identical in human alpha, beta, and gamma synuclein conjugated to diphteria toxoid|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||3 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||3 µg/mL|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18256 detects alpha, beta, and gamma Synuclein from human and rat samples.
Reconstitute with 100ul of distilled water. After reconstitution, store aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent.
Synuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimer's (AD), Parkinson's PD) and breast cancer. In AD, a peptide derived from alpha synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha synuclein accumulates in Lewy bodies. An allele of alpha synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.