Western blot analysis of phosphate-regulating neutral endopeptidase using 30µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a phosphate-regulating neutral endopeptidase polyclonal antibody (Product # PA5-21714) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 355 of PHEX (Uniprot ID#P78562)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21714 targets phosphate-regulating neutral endopeptidase in WB applications and shows reactivity with Human samples.
The PA5-21714 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 355 of PHEX (Uniprot ID#P78562).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.