Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells, 0.15 ug; Lane 1: proBNP antibody (Product # MA1-20622); Lane 2: proBNP antibody (Product # MA1-20626); Lane 3: proBNP antibody (Product # MA1-20886); Lane 4: proBNP antibody (Product # MA1-20584); Lane 5: proBNP antibody (Product # MA1-20631); Both proBNP antibody (Product # MA1-20584) and proBNP antibody (Product # MA1-20631) do not recognize glycosylated proBNP expressed in mammalian cells.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues E(13) T S G L Q E Q R N H L Q G K(27) of human proBNP.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
MA1-20886 detects proBNP in human samples.
MA1-20886 has been successfully used in ELISA and Western blot procedures.
The MA1-20886 immunogen is a synthetic peptide corresponding to the N-terminal residues E(13) T S G L Q E Q R N H L Q G K(27) of human proBNP.
In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa ""signal"" peptide and a 108 aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76 aa amino-terminal NT-proBNP and biologically active 32 aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.
BNP; brain type natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B; natriuretic protein