Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells, 0.15 ug; Lane 1: proBNP antibody (Product # MA1-20622); Lane 2: proBNP antibody (Product # MA1-20626); Lane 3: proBNP antibody (Product # MA1-20886); Lane 4: proBNP antibody (Product # MA1-20584); Lane 5: proBNP antibody (Product # MA1-20631); Both proBNP antibody (Product # MA1-20584) and proBNP antibody (Product # MA1-20631) do not recognize glycosylated proBNP expressed in mammalian cells.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide corresponding to residues E(13) T S G L Q E Q R N H L Q G K(27) of human proBNP.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-20626 detects proBNP in human samples.
MA1-20626 has been successfully used in ELISA and Western blot procedures.
The MA1-20626 immunogen is a synthetic peptide corresponding to residues E(13) T S G L Q E Q R N H L Q G K(27) of human proBNP.
In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa ""signal"" peptide and a 108 aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76 aa amino-terminal NT-proBNP and biologically active 32 aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
MA1-20626 was used in immunocytochemistry and western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin
|Sacco PA,McGranahan TM,Wheelock MJ,Johnson KR||The Journal of biological chemistry (270:20201)||1995|