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|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, full length, mouse sRANKL.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Neutralization (Neu)||0.001-1 µg/ml|
|Western Blot (WB)||0.1-0.2 mg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20150 detects sRANKL in mouse samples.
PA1-20150 has been successfully used in ELISA and Western blot procedures.
The PA1-20150 immunogen is recombinant, full length, mouse sRANKL.
TRANCE, also called RANKL (RANK ligand), OPGL (Osteoprotegrin ligand), and ODF (Osteoclast differentiation factor), is a type II transmembrane signaling protein of the TNF superfamily. The extracellular domain contains two potential N-linked glycosylation sites. Mouse TRANCE cDNA encodes a 316 amino acid protein with a calculated molecular mass of approximately 28 kDa. As a result of glycosylation, recombinant mouse TRANCE migrates as an approximately 36 kDa protein in SDS-PAGE. Human and mouse TRANCE share approximately 85% amino acid sequence identity. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. It is a key regulator of the immune system and of bone development and homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD254; Ly109l; ODF; OPG; OPG ligand; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; TNF-related activation-induced cytokine; TRANCE; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor-related activation-induced cytokine
Ly109l; ODF; OPG; OPGL; RANKL; Tnfsf11; Trance