Immunofluorescent analysis of splicing factor 1 showing staining in the nucleus of A375 cells. A375 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a splicing factor 1 polyclonal antibody (Product # PA5-28054) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 309 of Human splicing factor 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28054 targets splicing factor 1 in WB applications and shows reactivity with Human samples.
The PA5-28054 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 309 of Human splicing factor 1.
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.