Immunofluorescent analysis of steroidogenic acute regulatory protein isoform 1 showing staining in the mitochondria of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a steroidogenic acute regulatory protein isoform 1 polyclonal antibody (Product # PA5-21687) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 15 and 268 of STAR (Uniprot ID#P49675)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-21687 targets steroidogenic acute regulatory protein isoform 1 in WB applications and shows reactivity with Human and mouse samples.
The PA5-21687 immunogen is a recombinant fragment corresponding to a region within amino acids 15 and 268 of STAR (Uniprot ID#P49675).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Menaquinone-4 enhances testosterone production in rats and testis-derived tumor cells.
PA5-21687 was used in western blot to investigate the effect of menaquinone-4 on testosterone production in vitro and in vivo
|Ito A,Shirakawa H,Takumi N,Minegishi Y,Ohashi A,Howlader ZH,Ohsaki Y,Sato T,Goto T,Komai M||Lipids in health and disease (10:null)||2011|