Immunofluorescent analysis of ubiquinol-cytochrome c reductase Rieske iron-sulfur polypeptide 1 showing staining in the mitochondria of A549 cells. A549 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a ubiquinol-cytochrome c reductase Rieske iron-sulfur polypeptide 1 polyclonal antibody (Product # PA5-21420) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 274 of UQCRFS1 (Uniprot ID#P47985)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21420 targets ubiquinol-cytochrome c reductase Rieske iron-sulfur polypeptide 1 in WB applications and shows reactivity with Human and mouse samples.
The PA5-21420 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 274 of UQCRFS1 (Uniprot ID#P47985).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.