Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemistry was performed on human retina tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Visual Arrestin mouse monoclonal antibody (Product # MA3-814) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
|Tested species reactivity||Bovine, Goat, Human, Sheep, Pig, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Visual Arrestin isolated from Porcine Cells.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-814 detects Visual Arrestin from human, rat, porcine, bovine, ovine, and goat tissues.
MA3-814 has been successfully used in Immunohistochemistry, ELISA, and Western blotting procedures. By Western blot it detects a 37-kDa band representing Visual arrestin.
The MA3-814 antigen is Visual arrestin isolated from Porcine cells.
Functionally, arrestins uncouple G protein-coupled receptors through phosphorylation. Visual arrestin has been shown to interact directly with transducin, a GTP binding protein which initiates a cascade leading to visual signal transmission.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
48 kDa protein; arrestin 1; arrestin1; DKFZp686D1084; DKFZp686I1383; retinal S-antigen; retinal S-antigen (48 KDa protein); rod arrestin; rod photoreceptor arrestin; S-AG; S-antigen; S-antigen visual arrestin; S-antigen; retina and pineal gland (arrestin); S-arrestin
BOS_3864; RP47; S-AG; SAG; SAGMR; SANTI