Immunohistochemistry was performed on human retina tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Visual Arrestin mouse monoclonal antibody (Product # MA3-814) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
|Tested species reactivity||Bovine, Goat, Human, Sheep, Pig, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Visual Arrestin isolated from Porcine Cells.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA3-814 detects Visual Arrestin from human, rat, porcine, bovine, ovine, and goat tissues.
MA3-814 has been successfully used in Immunohistochemistry, ELISA, and Western blotting procedures. By Western blot it detects a 37-kDa band representing Visual arrestin.
The MA3-814 antigen is Visual arrestin isolated from Porcine cells.
Functionally, arrestins uncouple G protein-coupled receptors through phosphorylation. Visual arrestin has been shown to interact directly with transducin, a GTP binding protein which initiates a cascade leading to visual signal transmission.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Analysis of antigenic determinants of retinal S-antigen with monoclonal antibodies.
MA3-814 was used in immunoprecipitation, immunohistochemistry and western blot to characterize retinal S-antigen antigenic epitopes
|Banga JP,LeRoy F,Suleyman S,Kasp E,Brown E,Dumonde D||Investigative ophthalmology and visual science (29:12)||1988|