Immunofluorescent analysis of zinc finger protein 45 showing staining in the nucleus of HCT116 cells. HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a zinc finger protein 45 polyclonal antibody (Product # PA5-27906) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 213 and 629 of Human zinc finger protein 45|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27906 targets zinc finger protein 45 in WB applications and shows reactivity with Human samples.
The PA5-27906 immunogen is recombinant fragment corresponding to a region within amino acids 213 and 629 of Human zinc finger protein 45.
May be involved in transcriptional regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.