Targeted metabolomics profiling and quantitation solutions

Targeted profiling and quantitation solutions

Identified biomarkers from either untargeted metabolomics experiments, or hypothesis-driven from the literature, can be verified or validated where usually a limited number of compounds are quantified in a large set of samples.

For highest selectivity and confirmation, HRAM Orbitrap mass spectrometry is the technology of choice for verification of putative biomarkers. Moving through to high-throughput quantitation, SRM analysis on the TSQ triple quadrupole mass spectrometers is the solution of choice.

Popular metabolomics products

Standardized and quantitative HRAM LC-MS metabolomics workflow

Targeted metabolomics offers researchers the possibility to quantify selected metabolites. The AbsoluteIDQ® p400 HR Kit is the first standardized solution for broad metabolic and lipid profiling developed for Thermo Scientific high-resolution, accurate mass (HRAM) Orbitrap MS. It covers hundreds of metabolites while providing quantification, reproducibility, and high throughput. The kit offers the opportunity to analyze various biological matrices from multiple species.

Sample preparation

First steps

Samples are registered in the MetIDQ™ software (integral part of the kit). The test mix is run to ensure suitable system conditions.

Loading

Samples and reagents are pipetted onto the kit plate

Derivatization

Amino acids and biogenic amines are derivatized to help ensure a reliable analysis.

Extraction

Metabolites are extracted with organic buffer and diluted in two steps for subsequent mass spectrometric analysis.

LC-MS and FIA-MS Analysis

Data analysis

Sample preparation

First steps

Samples are registered in the MetIDQ™ software (integral part of the kit). The test mix is run to ensure suitable system conditions.

Loading

Samples and reagents are pipetted onto the kit plate

Derivatization

Amino acids and biogenic amines are derivatized to help ensure a reliable analysis.

Extraction

Metabolites are extracted with organic buffer and diluted in two steps for subsequent mass spectrometric analysis.

LC-MS and FIA-MS Analysis

Data analysis


Featured webinar

Standardized Targeted Metabolic Profiling Assay using the Q Exactive and the AbsoluteIDQ kit designed for HRAM MS.