Quickly confirm intact protein molecular weight and determine glycoform heterogeneity of biopharmaceuticals. High-resolution accurate-mass (HRAM) mass spectrometry is a vital tool for intact protein mass analysis, enabling a direct comparison of biotherapeutics products throughout the drug pipeline.
Intact Protein Analysis Workflows
Confident protein characterization methods
Get the latest information on the analysis of biotherapeutic proteins such as monoclonal antibodies (mAbs) in their intact form. UHPLC is ideal for identifying modifications such as charge variants, as well as higher order structures through aggregation. Use our broad range of leading column chemistries for these and other intact analyses by liquid chromatography (LC). For rapid and precise accurate mass determination, try our uniquely powerful Thermo Scientific Orbitrap-based mass spectrometers (MS) for analysis of both denatured and native intact proteins.
Perform robust analysis of charge variants in monoclonal antibodies. The charge heterogeneity of a biotherapeutic protein strongly influences the stability and efficacy of the drug. Learn about our range of pH buffers, ion exchange chromatography (IEX) column chemistries and UHPLC instrumentation required for full charge variant profiling.
Protein aggregation gives rise to higher order structures which can significantly affect the efficacy of biologic drugs and cause harmful side effects. Discover the leading size exclusion chromatography (SEC) solutions available to ensure confident characterization of sub-visible protein aggregates in biotherapeutic formulations.
Perform selective digestion of mAbs to antibody fragments and simplify your analysis. Find products for chromatography of protein fragments and see how the resolving power of the Orbitrap mass analyzers ensures resolution of subunits of proteins and facilitates top/middle down sequencing.
Intact protein analysis under native mass spectrometry conditions preserves structurally-critical non-covalent binding between antibody chains. Providing the ideal technique to aid characterization of antibody drug conjugates (ADCs) and other heterogeneous antibody samples. Find out more about our routine native analysis columns and on-line size exclusion chromatography (SEC).
Determination of protein concentration, known as titer, is widely used in the biotechnological protein production process. Choose from our wide range of affinity columns and resins from discovery to QA/QC for titer measurement. Our range of protein-A protein purification columns can automate biopharmaceutical protein isolation ahead of additional characterization steps, such as charge variant profiling.
Prof. Alexander Makarov takes us through the evolution of Obitrap technology for the analysis of biotherapeutics.
Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using the Q Exactive BioPharma platform of mass spectrometers.