Androgen Receptor Antibodies, PA1-110

Androgen receptors are transcription factors belonging to the nuclear receptor superfamily. They are expressed in bone marrow, mammary gland, brain, hair follicles, cardiovascular, prostate, testicular and muscle tissues where they exist as dimers coupled to accessory proteins, which are shed upon ligand binding.

When activated by ligand binding, androgen receptors stimulate the transcription of androgen responsive genes. Androgen receptors play an essential role in male development including development of primary and secondary male sexual characteristics during embryogenesis and puberty, maintenance of sexual function, and may play a causal role in aggressive behavior.

Congenital mutations in androgen receptors are linked with androgen insensitivity syndromes, virility problems, and spinal and bulbar muscular atrophy. Changes in androgen receptor expression are also associated with prostate cancer. Androgen receptor antibodies aid in the detection and study of the androgen receptor and its functions. Quality Invitrogen androgen receptor antibodies are available for your research needs.

Popular androgen receptor antibodies

Western blot analysis of androgen receptor

Western blot analysis of androgen receptor was performed by loading 25 µg of T47D (lane 1), LNCaP (lane 2) and NIH-3T3 (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with an androgen receptor monoclonal antibody (Cat. No. MA1-150) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Cat. No. 32132). Results show a band at ~110kDa in T47D and LNCaP lysates.

 


Immunofluorescent analysis of androgen receptor

Immunofluorescent analysis of androgen receptor (green) in LNCaP (right panel) and negative control U2OS cells (left panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Cat. No. 37525) for 15 minutes at room temperature. Cells were probed with an androgen receptor monoclonal antibody (Cat. No. MA5-13426) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Cat. No. 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin (Cat. No. 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Cat. No. 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.


Immunohistochemistry analysis of androgen receptor

Immunohistochemistry analysis of androgen receptor showing staining in the cytoplasm and nucleus of paraffin-treated human prostate tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with an androgen receptor polyclonal antibody (Cat. No. PA1-110) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.


Annotated product references

Cat. No. MA1-150 was used in western blot to investigate the effect of androgen on gonadotropin-releasing hormone secretion and gene expression. Shakil T, Hoque AN, Husain M et al. (2002) Differential regulation of gonadotropin-releasing hormone secretion and gene expression by androgen: membrane versus nuclear receptor activation. Mol Endocrinol 16:2592–2602.

Cat. No. MA5-13426 was used in immunohistochemistry to study a novel androgen receptor mutation and its potential role in testicular dysgenesis syndrome. Lottrup G, Jørgensen A, Nielsen JE et al. (2013) Identification of a novel androgen receptor mutation in a family with multiple components compatible with the testicular dysgenesis syndrome. J Clin Endocrinol Metab 98:2223–2229.

Cat. No. PA1-110 was used in immunohistochemistry to study testosterone regulation of sex steroid-related mRNAs and dopamine-related mRNAs in adolescent male rat substantia nigra. Purves-Tyson TD, Handelsman DJ, Double KL et al. (2012) Testosterone regulation of sex steroid-related mRNAs and dopamine-related mRNAs in adolescent male rat substantia nigra. BMC Neurosci 13:95.