We've collected protocols for all of our transfection reagents (21 in total!) in one spot. Once you've located the reagent that matches your requirements from the list, the protocol is just a click away.
Finding that optimal match
Whether for protein expression, gene expression modulation, or genome editing, efficient, effective transfection relies on an optimal match between the cultured cells, the transfection reagent or equipment, and post-transfection care and maintenance. Get the information you need to get the results you're aiming for.
Download this free guide that outlines a collection of superior transfection and TALs/CRISPR genome engineering products designed to work to gether to help answer important scientific questions faster and with less effort.
This application note provides an overview for the use of Lipofectamine™ MessengerMAX™ reagent is used for high-efficiency transfection of neuronal cell models, enabling improved results and simplified workflows for applications such as genome editing. We also compare Lipofectamine MessengerMAX reagent to other gene delivery solutions and highlight the advantages and drawbacks of each approach.
After you've located your cell line from the extensive list, you'll be able to see the recommended transfection reagent and a link to the protocol.
Performing electroporation with the Neon Transfection System
Learn about the flexibility, simplicity, and versatility of the Neon™ Transfection system--the next-generation electroporation device for highly efficient transfection of primary cells, stem cells and difficult-to-transfect cells.
Lipofectamine MessengerMAX mRNA Transfection Reagent
See why neurons and primary cells can be harder to transfect than other cell types and how Lipofectamine™ MessengerMAX™ mRNA Transfection Reagent can achieve up to five times the transfection efficiency of DNA reagents in these difficult-to-transfect cells.
Browse cell culture & transfection learning center content by collection (resource type)—application notes, case studies, videos, webinars, and white papers.
- Lipofectamine™ transfection reagent selection guide
- Transfecting neural cells using the Neon™ Transfection System
- CRISPR transfection selection guide
- Cell Culture Application Notes
- Blog: Advancing Cell Culture
- Webinars: Cell Culture Cafe On-Demand Webinars
- Protein Expression Handbook
- Select your Gibco™ Opti-MEM™ media
- Gibco™ Cell Culture Companion App
- Thermo Scientific™ Lab Products App Library