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Resources for Reverse Transcriptases

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Resources for SuperScript V Reverse Transcriptase

Manuals and protocols

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  • App note: FFPE (Coming soon)
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  • App note: Immune Sequencing (Coming soon)

Videos

SuperScript IV Reverse Transcriptases: Citations

SuperScript IV Reverse Transcriptases are highly cited in the several peer reviewed research publications. In three years, between 2022 and 2024, it has been cited over in 10K publications.

Use

Reference

Detect cancer biomarkers from extracellular vesicles by ddPCR (digital droplet).

Lee KY, Beatson EL, Knechel MA, et al. (2024). Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3. J Cancer 15(3):615–622.
doi: 10.7150/jca.90836. PMID: 38213719.

cDNA synthesis for preparation of a Poly(A)-seq library and for nested RT-PCR to study changes in UTR length.

Gabel AM, Belleville AE, Thomas JD, et al. (2024). Multiplexed screening reveals how cancer-specific alternative polyadenylation shapes tumor growth in vivo. Nat Commun 15:959.
doi: 10.1038/s41467-024-44931-x. PMID: 38302465.

Synthesize cDNA from FFPE samples for ddPCR.

Look T, Puca E, Bühler M, et al. (2023). Targeted delivery of tumor necrosis factor in combination with CCNU induces a T cell-dependent regression of glioblastoma. Sci Transl Med 15(697).
doi: 10.1126/scitranslmed.adf2281. PMID: 37224228.

Use

Reference

Reverse transcribe viral RNA to cDNA prior to circularization, RCA, and nanopore sequencing.

Misu M, Yoshikawa T, Sugimoto S (2023). Rapid whole genome sequencing methods for RNA viruses. Front Microbiol 14.
doi: 10.3389/fmicb.2023.1137086. PMID: 36910229.

Reverse transcribe avian viral RNA prior to amplification and library preparation.

Narvaez SA, Harrell TL, Oluwayinka, et al. (2023). Optimizing the Conditions for Whole-Genome Sequencing of Avian Reoviruses. Viruses 15(9):1938.
doi: 10.3390/v15091938. PMID: 37766345.

Substituted SuperScript IV Reverse Transcriptase for the reverse transcription step in an Illumina library preparation workflow, improving sequencing depth.

Soufi EG, Jorio DL, Gerber Z, et al. (2024). Highly efficient and sensitive membrane-based concentration process allows quantification, surveillance, and sequencing of viruses in large volumes of wastewater. Water Res 249.
doi: 10.1016/j.watres.2023.120959. PMID: 38070350.

Measure concatemeric cDNA formation upon phage infection. This cDNA encodes a toxic protein.

Wilkinson ME, Li D, Gao A, et al. (2024). Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA. Science 386(6717).
doi: 10.1126/science.adq3977. PMID: 39208082.

Synthesize cDNA from HIV RNA isolated from patients. The amplified DNA was analyzed by gel electrophoresis and NGS, and cloned for Sanger sequencing.

Esmaeilzadeh E, Etemad B, Lavine CL, et al. (2023). Autologous neutralizing antibodies increase with early antiretroviral therapy and shape HIV rebound after treatment interruption. Sci Transl Med 15(695).
doi: 10.1126/scitranslmed.abq4490. PMID: 37163616.

Use

Reference

Characterize monoclonal antibodies from B cells. Developed a switching mechanism at the 5′ ends of the RNA transcript (SMART)-based method for amplifying IgV genes from single rhesus macaque memory B cells to capture paired immunoglobulin heavy and light chains.

Weinfurter JT, Bennett SN, Reynolds MR (2024). A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells. J Immunol Methods 525:113602.
doi: 10.1016/j.jim.2023.113602. PMID: 38103783.

Synthesize cDNA for RT-qPCR to study gene expression changes following CRISPR knockout of the transcription factor Mef2d.

Szeto ACH, Clark PA, Ferreira ACF, et al. (2024). Mef2d potentiates type-2 immune responses and allergic lung inflammation. Science 384(6703).
doi: 10.1126/science.adl0370. PMID: 38935708.

Synthesize cDNA to measure the expression of Epstein–Barr virus transcripts.

Zhang TT, Cheng RY, Ott AR, et al. (2024). BCR signaling is required for posttransplant lymphoproliferative disease in immunodeficient mice receiving human B cells. Sci Transl Med 16(742).
doi: 10.1126/scitranslmed.adh8846. PMID: 38598616.

Synthesize cDNA from human B cells. The cDNA was amplified and sequenced.

Grigoryan L, Feng Y, Bellusci L, et al. (2024). AS03 adjuvant enhances the magnitude, persistence, and clonal breadth of memory B cell responses to a plant-based COVID-19 vaccine in humans. Sci Immunol 9(94).
doi: 10.1126/sciimmunol.adi8039. PMID: 38579013.

cDNA synthesis for RT-qPCR using patient samples.

Morot J, Del Duca E, Chastagner M, et al. (2023). Hyperactivation of the JAK2/STAT5 signaling pathway and evaluation of baricitinib treatment among patients with eosinophilic cellulitis. JAMA Dermatol 159(8):820–829.
doi: 10.1001/jamadermatol.2023.1651. PMID: 37342057.

Synthesize cDNA from human B cells. The cDNA was amplified and sequenced.

Dejoux A, Zhu Q, Ganneau C, et al. (2024). Rocuronium-specific antibodies drive perioperative anaphylaxis but can also function as reversal agents in preclinical models. Sci Transl Med 16(764).
doi: 10.1126/scitranslmed.ado4463. PMID: 39259810.

Use

Reference

Study mRNA expression profiles in tissue and identify a unique urea transport pathway in the spiny dogfish shark.

Cutler CP, Omoregie E, Ojo T (2024). UT-1 Transporter Expression in the Spiny Dogfish (Squalus acanthias): UT-1 Protein Shows a Different Localization in Comparison to That of Other Sharks. Biomolecules 14(9):1151.
doi: 10.3390/biom14091151. PMID: 39334917.

Used SuperScript IV Reverse Transcriptase with a low-input, difficult RNA sample isolated from insect antennae.

Johny J, Nihad M, Alharbi HA, et al. (2024). Silencing sensory neuron membrane protein RferSNMPu1 impairs pheromone detection in the invasive Asian Palm Weevil. Sci Rep 14(1):16541.
doi: 10.1038/s41598-024-67309-x. PMID: 39019908.

Synthesize cDNA from RNA isolated from yeast cells to measure gene expression.

Montrose K, Lac DT, Burnetti AJ, et al. (2024). Proteostatic tuning underpins the evolution of novel multicellular traits. Sci Adv 10(10).
doi: 10.1126/sciadv.adn2706. PMID: 38457507.

Use

Reference

Convert RNA to cDNA prior to RT-qPCR to measure the relative expression levels of target genes in wound healing and angiogenesis assays.

Rapp J, Ness J, Wolf J (2024). 2D and 3D in vitro angiogenesis assays highlight different aspects of angiogenesis. Biochim Biophys Acta Mol Basis Dis 1870(3).
doi: 10.1016/j.bbadis.2023.167146. PMID: 38244944.

Synthesize cDNA for the study of RNA splicing. Products were analyzed by agarose gel electrophoresis, qPCR, and Sanger sequencing.

Žedaveinytė R, Meers C, Le HC, et al. (2024). Antagonistic conflict between transposon-encoded introns and guide RNAs. Science 385(6705).
doi: 10.1126/science.adm8189. PMID: 38991068.

Synthesize cDNA from CRISPR-modified cells for RNA-seq.

Dudnyk K, Cai D, Shi C, Xu J, et al. (2024). Sequence basis of transcription initiation in the human genome. Science 384(6694).
doi: 10.1126/science.adj0116. PMID: 38662817.

For Research Use Only. Not for use in diagnostic procedures.