iWestern Workflow
Maximize results and minimize hands-on time with our innovative western blotting bundle

Streamline your western blotting workflow by empowering your lab with the Invitrogen iWestern Workflow solution. Our iWestern Workflow is an inventive take on the decades long problem – getting the desired results from what can be the elusive, time-consuming western blot.

Designed to address processing efficiency, reproducibility, and robustness of results, the iWestern Workflow helps to enable exceptional western blot results with minimal hands-on time. With the iWestern Workflow, your life in the lab becomes easier, and less time-consuming, allowing time for you to focus on what matters most—your data.

At the core of the iWestern Workflow are four innovative western blotting products designed around efficiency, reproducibility, and optimized to deliver the performance you expect from us. Learn more about these innovative products below and start building your iWestern bundle.

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Compare data obtained using iWestern workflow and Manual western blotting

The iWestern workflow enables the detection of HDAC1 in HeLa lysate in appoximately 4 hours vs. 18 hours using the manual western workflow. Two-fold serial dilutions (starting with 20 ug of HeLa lysate) were loaded into wells of tris-glycine gels. The proteins on the gels were transferred to nitrocellulose membranes and detected using SuperSignal West Pico PLUS chemiluminescent substrate.

  iWestern Time Manual Time
Western Steps iWestern time Manual time
Sample Prep/Gel Electrophoresis 50 min 90 min
Transfer 7 min 75 min
Blocking 180 min 60 min
Primary Incubation 720 min
Washes 60 min
Secondary Incubation 60 min
Substratre Incubation 5 min 5 min
Target Detection 2 min 5 min
Total time 244 minutes (app. 4 hours) 1,075 minutes (app. 18 hours)

Calculate time savings between iWestern workflow and Manual western blotting

Don’t get stuck “Blot-sitting” with the manual processing of your blots. Western empower your lab with the Invitrogen iWestern workflow today!

Save precious time using the iWestern workflow

Fig. 1. Time to western results: Traditional vs. iWestern workflow.

Compare data obtained using iWestern workflow and Manual western blotting

The iWestern workflow enables the detection of HDAC1 in HeLa lysate in appoximately 4 hours vs. 18 hours using the manual western workflow. Two-fold serial dilutions (starting with 20 ug of HeLa lysate) were loaded into wells of tris-glycine gels. The proteins on the gels were transferred to nitrocellulose membranes and detected using SuperSignal West Pico PLUS chemiluminescent substrate.

  iWestern Time Manual Time
Western Steps iWestern time Manual time
Sample Prep/Gel Electrophoresis 50 min 90 min
Transfer 7 min 75 min
Blocking 180 min 60 min
Primary Incubation 720 min
Washes 60 min
Secondary Incubation 60 min
Substratre Incubation 5 min 5 min
Target Detection 2 min 5 min
Total time 244 minutes (app. 4 hours) 1,075 minutes (app. 18 hours)

Calculate time savings between iWestern workflow and Manual western blotting

Don’t get stuck “Blot-sitting” with the manual processing of your blots. Western empower your lab with the Invitrogen iWestern workflow today!

Save precious time using the iWestern workflow

Fig. 1. Time to western results: Traditional vs. iWestern workflow.

With an intelligently engineered side-by-side design, the Invitrogen Mini Gel Tank provides a forward-facing well configuration for easier sample loading and the simultaneous visualization of both gels, while the white-colored tank stand provides contrast to improve the monitoring of pre-stained markers during electrophoresis. Compatible with over 180 gels of different formats and gel chemistries, the Mini Gel Tank provides the flexibility to choose the best gel for the job.

The standard iWestern Workflow Bundle includes our Bolt Gel Welcome Pack, utilizing Bis-Tris gel chemistry designed for optimal western blotting performance, but you can also choose a gel welcome pack utilizing a chemistry of your choice with our custom bundle option.

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The Invitrogen iBlot 2 Dry Blotting System is our ultimate western blot transfer device, delivering high performance and convenience. A unique, innovative dry blotting system, iBlot 2 utilizes pre-assembled transfer stacks, with transfer buffer incorporated into gel matrices, so there is no need to prepare messy transfer buffers, and there is minimal post-transfer cleanup required. The short distance between electrodes, along with high field strength and current, reduces transfer time to 7 minutes. Just insert your gel and go!

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Two devices, one simple technology for western blot processing. The Invitrogen iBind Western Systems are simple, non-powered devices that automate many of the tedious, routine western blot processing steps. The original iBind Western Device accommodates the processing of one mini blot at a time, while the iBind Flex Western Device accommodates the processing of up to one midi blot, two mini blots, or up to six vertically cut strips. The entire immunoblotting process is complete in less than 3 hours, uses up to 80% less primary antibody than traditional methods, and because processing is automated, blot-to-blot consistency can be improved.

Our standard iWestern Workflow bundle comes with the iBind Western System, but if increased throughput is needed, choose our iBind Flex Western System.

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Complete with a powerful 9.1-megapixel camera, proprietary Smart Exposure acquisition technology, Thermo Fisher Cloud connectivity, and a suite of automated features, the Invitrogen iBright Imaging Systems enable customers to capture and analyze western blots and gels faster and easier than before.

An intelligent blend of art and power, we’ve designed the iBright Imaging Systems with a clean, simple interface that enables users to easily harness the full capabilities of the platform. Two models are available—the iBright CL1000 model, capable of imaging chemiluminescent western blots in addition to stained protein and nucleic acid gels, and the iBright FL1000 model, featuring the same imaging modes as the iBright CL1000, yet also offering 5-channel fluorescent blot imaging capability in both visible and near-IR channels.

The standard iWestern Workflow includes the iBright FL1000 Imaging System.

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iWestern Workflow

Ready to streamline your western blotting experience with the iWestern Workflow?

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