Sep 22, 2016 - Sep 22, 2016
Cell culture cafe

Cell Culture Café Webinars

Welcome to the Cell Culture Café, a science-based community where techniques, tips, and questions concerning cell culture are presented and discussed. Via interactive webinars, our cell culture experts will explore topics ideally suited to your biggest concerns in the lab.

If you have a suggestion for a future webinar, let us know! We welcome your feedback and want to learn what topics interest you.

Presenter: Kim Knepper, Global Application Specialist, Thermo Fisher Scientific  

Next Webinar:

Thursday, September 22, 2016
Session #1: 8:00 am EDT /1:00 pm CET / 5:30 pm IST
Session #2: 1:00 pm EDT / 6:00 pm CET / 10:30 pm IST

Title: ULTRAPURE WATER AND CELL CULTURE: Know why, what and how for proper water system maintenance

Overview of Webinar: Ultrapure water is an important staple to the cell culture lab. The water system is expected to deliver water that is free of impurities, of consistent quality and always available upon demand. This webinar investigates the different types of impurities in water and how water systems remove these critical impurities. We will also review guidelines for properly maintaining the lab water system to ensure successful cell culture.  

Topics covered:

  • Review the six impurities found in lab water including one that is critical to cell culture
  • Investigate 7 common consumables to remove these impurities and  their critical role to purify ultrapure water
  • Establish guidelines for setting up standard maintenance for ultrapure water systems in life science labs

Featured Cell Culture Café on-demand webinars

Title On-Demand

In Search of the Perfect Pellet: Recommendations for Cell Culture Centrifugation

The centrifuge is a crucial but often overlooked component within the cell culture workflow. Cultured mammalian cells are extremely delicate and require gentle centrifugation processing to maximize viable yield. Join us as we discuss important centrifuge considerations, to help increase efficiency and ergonomics, while reducing the risk of contamination. We will examine ways to help achieve optimal pellets and how to improve reproducibility between runs for high-quality results.

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The Art of Keeping Cool and Maintaining Cell Viability

Cryopreservation is a critical step in the cell culture workflow. Correctly done, it can yield thousands of viable cells for downstream use. In this webinar we examined key aspects of cryopreservation, including choosing the correct sample storage vial, ideal storage temperatures and storage solutions, and control rate cooling before final freezing.

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Best Practices in Cell Counting for Accurate and Reproducible Data

Accurate cell counting is a critical component for many biological applications. Without precise and reproducible cell counting and viability data, it is very difficult to determine if the root cause of downstream observations are due to cell culture and sample preparation or the intended experimental variables being tested. In this webinar, we will discuss best practices for manual and automated cell counting and the effect of cell preparation on viability measurements.

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Understanding All Incubation Parameters for Sensitive Cell Growth

In advanced biological applications such as pharmaceutical testing, regenerative medicine/cell therapy, and modeling of cancer, immunology, neurology etc., the integrity and health of cells are proportionally more important. To best support sensitive cells and to ensure they give accurate responses predictive of in vivo conditions, new CO2 incubators designed for 21st century cell culture offer better culturing environments than previously available. This presentation will help you understand how all incubation parameters work together to support cell health.

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From the Hood to the Microscope: Revolutionizing Cell-Based Imaging

Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow—from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging.

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Versatile is Smart! The Power Behind Culturing Cells on a Porous Membrane

There are times when your cell-based research calls for more than your standard cell culture vessels and dishes. The porous membrane and unique structure of cell culture inserts enables the versatility you need by allowing the attached surface of your cells to be exposed to growth media and different conditions simultaneously— providing easier methods for some procedures, such as cultures that require an air-liquid interface and media feeding. In this presentation, we examine the possible uses and adaptability of cell culture inserts and review a case study using cell culture inserts to produce an artificial skin growth model at air-liquid interface.

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Optimizing Results in Your Downstream Imaging Applications

Get the clear picture on your imaging applications workflow – from culturing and seeding your cells to image acquisition and data analysis - and how you can significantly improve your imaging results downstream by merely choosing the right culture vessel. In order to highlight the critical importance of using the right culture surface and format, we will present a case study of optimized neuron growth in chamber slides, in which we examine the correlation between neuron cell adhesion and multiple chamber slide surface modifications.

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Eliminate the Threat: Cleaning and Contamination in your Biological Safety Cabinet

Contamination is the ever present threat to good science and your hard work. This webinar provides important points that will help you work with your biological safety cabinet more effectively to prevent contamination in your cell culture lab.

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Browse the Cell Culture Café on-demand webinar library