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Performance-defining cryo-EM components and software
Early 200 kV cryo-transmission electron microscopes were not widely adopted for high-resolution cryo-EM because they struggled to deliver the throughput and usability needed for detailed structural reconstructions. As a result, previous generations of 200 kV cryo-TEMs were used primarily for sample screening before imaging on 300 kV instruments.
Today, the Thermo Scientific Glacios 3 Cryo-TEM is fully integrated with advanced components and software that enable high resolution, throughput, and usability in 200 kV imaging, making it an effective system for both single particle analysis and cryo-ET across a range of biological applications, from novel research to drug discovery and design.
From blobology to high-resolution structure determination
The improvement in capabilities of 200 kV cryo-TEM illustrated with rabbit muscle aldolase.
Falcon 4i Direct Electron Detector for fast, high-quality imaging
The Thermo Scientific Falcon 4i Direct Electron Detector helps deliver fast imaging and exceptional image quality, making it an ideal choice for high-throughput cryo-EM workflows. Its fast frame rate and reduced overhead enable efficient data collection, while its high detective quantum efficiency (DQE) helps ensure outstanding image quality across all spatial frequencies. The innovative electron event representation (EER) mode boosts DQE at high resolutions, supporting super-resolution imaging and lossless data compression without up-front fractionation. The Falcon 4i Detector integrates seamlessly into automated workflows and supports unattended acquisition through Thermo Scientific software. Flexible scripting also facilitates third-party software integration, accelerating cryo-EM research through excellent data quality.
Dedicated, AI-powered software solutions to increase throughput and data quality
Advances in software have transformed both data acquisition and analysis in cryo-EM, driving the field from its early, manual methods to today's high-throughput, high-resolution workflows. Modern, AI-powered Thermo Scientific software enables automated, unattended acquisition, optimizing microscope performance, and maximizing efficiency.
For analysis, advances in image processing algorithms, such as motion correction, particle picking, and 3D reconstruction, have significantly improved speed, accuracy, and resolution. Overall, these software innovations have made cryo-EM more accessible, scalable, and powerful, helping to accelerate discoveries in structural biology.
Connected cryo-EM workflows
Vacuum Capsule enhances workflows that use multiple cryo instruments
The Thermo Scientific Vacuum Capsule is designed to avoid ice deposition during sample transfer by keeping cryogenic specimens within an enclosed reservoir. It enables safe, easy sample transfer, thereby improving the overall success rate of cryo-EM workflows such as cryo-ET lamella preparation, or the transfer of single particle analysis samples between 200 kV and 300 kV instruments.
The Vacuum Capsule is fully compatible with Thermo Scientific cryo-TEMs that have an Autoloader system, including the Glacios 3 Cryo-TEM.
Cryo-ET supported by TomoGrid compatibility
Cryo-electron tomography relies on precise alignment of the delicate cryogenic lamella during sample loading. Proper positioning helps keep the region of interest within the tilt range and field of view throughout data collection. The Glacios 3 Cryo-TEM supports TomoGrids, specialized sample carriers designed to maintain the correct orientation of the lamella relative to the TEM tilt axis. This minimizes data loss, improves tilt-series quality, reduces mechanical stress on the lamella, and ultimately increases the success rate of high-resolution reconstructions.
Cryo-TEM component and software resources
For Research Use Only. Not for use in diagnostic procedures.