Talk TitleSpeakerAbstract Duration
It’s all in the prep!Audrey Lim, Ph.D.It is no secret that preparation is the key to success – High-quality qPCR data begins with the synthesis of high-quality cDNA. Gene expression assay optimization should begin from RNA isolation to cDNA synthesis, as different sample types and target regions present different challenges. In this session, we will discuss some practical considerations around improving sample preparation methods and selecting the right reverse transcriptase to set you up for success.20 minutes
Best practices for running impactful gene expression studies. Where can we go wrong?Karolina Setyowati, Ph.D.Real-time PCR is a powerful tool that has remained as the gold standard for gene expression studies in various applications. Although easy to perform, planning a real-time PCR experiment can be a complex process as there is a multitude of variables to consider and optimize. In fact, the quality of your real-time PCR data is only as good as the quality of your assay, experimental setup as well as data analysis. This webinar discusses the fundamentals of real-time PCR technology, important considerations to keep in mind as well as the challenges and vulnerabilities of gene expression experiments, and how to address them. 25 minutes

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