Caveolae Marker Antibodies, PA1-066

Caveolae are small, specialized areas of the plasma membrane. These organelles are involved in sequestering a variety of protein and lipid molecules. Three caveolin proteins (caveolin-1, caveolin-2, and caveolin-3) are the primary components of caveolae, but their distribution varies with tissue type.

Caveolins 1 and 2 have similar expression in fibroblasts, differentiated adipocytes, endothelial cells, and smooth and skeletal muscle. Caveolin-3 appears to be expressed only in muscle tissues. When internalized, caveolae can fuse with early endosomes, which then may be recycled back to the plasma membrane or sent on for degradation.

Caveolae are involved in many important biological functions including lipid metabolism, vesicular trafficking, signal transduction, endocytosis, exocytosis, cell adhesion, and apoptosis. They also play a role in neurodegenerative disease, oncogenesis, and the uptake of some viruses and pathogenic bacteria.

Caveolae marker antibodies detect proteins specific to the caveolae and can aid in the study of the structure and function of the caveolae. Caveolae marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the caveolae. Quality Invitrogen caveolae marker antibodies are available for a variety of research needs.

Featured product data

Immunofluorescent analysis of caveolin 3 in C2C11 Cells

Immunofluorescent analysis of caveolin 3 in C2C11 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a caveolin 3 polyclonal antibody (Cat. No. PA1-066) at a dilution of 1:20 overnight at 4°C, washed with PBS and incubated with a DyLight™ 488-conjugated secondary antibody (Cat. No. 35503). Caveolin 3 staining (green), F-actin staining with phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60x magnification.

Immunohistochemistry on normal biopsies of deparaffinized rat lymph node tissue.

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Caveolin 1 (Cat. No. MA3-600) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Flow cytometry analysis of caveolin 1 in CHO cells (green) compared to an isotype control (blue)

Flow cytometry analysis of Caveolin 1 in CHO cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 minutes at room temperature and incubated with a Caveolin 1 monoclonal antibody (Cat. No. MA3-600) at a dilution of 1:20 for 60 minutes at room temperature. Cells were then incubated for 40 minutes at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Annotated product references

Cat. No. PA1-064 was used in immunohistochemistry to investigate the involvement of androgen and oxytocin receptor in the pathogenesis of benign prostate hyperplasia. Anatomia, histologia, embryologia (Oct 2008; 37: 325) "Colocalization of androgen binding protein, oxytocin receptor, caveolin 1 and proliferation marker p21 in benign prostate hyperplasia." Sendemir E,Herbert Z,Sivukhina E,Zermann DH,Arnold R,Jirikowski GF

Cat. No. PA1-066 was used in immunohistochemistry, immunoprecipitation, and western blot to investigate the mechanism for the involvement of caveolin-3 in the pathogenesis of muscle caveolinopathy. The international journal of biochemistry & cell biology (May 2011; 43: 713) "Caveolin-3 is a direct molecular partner of the Cav1.1 subunit of the skeletal muscle L-type calcium channel." Couchoux H,Bichraoui H,Chouabe C,Altafaj X,Bonvallet R,Allard B,Ronjat M,Berthier C

Cat. No. MA3-600 was used in western blot to investigate the role of aquaporin-4 and its oligmers in the formation and function of T-tubules of skeletal myofibers. Experimental cell research (Jan 2011; 317: 20) "Aquaporin-4 water channel oligomers are associated with the transverse tubules of skeletal myofibers." Kaakinen M,Zelenin S,Metsikkö K