Plasma Membrane Marker Antibodies
The plasma membrane is a selectively permeable phospholipid bilayer that surrounds the cytoplasm of cells, separating the intracellular components from the extracellular environment. It is primarily composed of proteins and lipids and is instrumental in signaling pathways. The plasma membrane also anchors the cytoskeleton, giving the cell shape, and serves as an attachment point for the extracellular matrix and surrounding cells to help them group together to form tissues.
Plasma membrane marker antibodies detect proteins specific to the plasma membrane and can aid in the study of the morphology and functions of the plasma membrane. Plasma membrane marker antibodies can also help elucidate the role a protein may play in tasks that are centered in the plasma membrane such as transporter, receptor, anchor, enzyme, etc.
Each Invitrogen plasma membrane marker antibody is validated for use in applications such as ELISA, western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and immunoprecipitation.
Plasma membrane antibody targets
Featured product data
Flow cytometry analysis of PSD95 in U87-MG cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1–5 x 106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 minutes at room temperature and incubated with a PSD95 monoclonal antibody (Cat. No. MA1-046) at a dilution of 2 µg/test for 60 minutes at room temperature. Cells were then incubated for 40 minutes at room temperature in the dark using a DyLight 488−conjugated goat anti-mouse IgG (H+L) (Cat. No. 35503) secondary antibody and resuspended in PBS for FACS analysis.
Sandwich ELISA analysis of human Interferon Gamma (IFNG) was performed using a Human Interferon Gamma Colorimetric ELISA kit by loading 50 ul per well of Interferon Gamma Recombinant Protein (Cat. No. RIFNG100) in quadruplicate at 1000, 400, 160, 64, 25.6, and 0 pg/ml across a 3 ug/ml mouse anti-human IFNG (Cat. No. M700A) pre-coated plate and incubating for 2 hours at room temperature. The plate was washed, then incubated with 50 ul per well of a mouse anti-human IFNG biotinylated antibody (Cat. No. M701B) in quadruplicate at a concentration of 0.05 ug/ml for 2 hours at room temperature. The plate was washed and incubated with 100 ul per well of Streptavidin-HRP (Cat. No. N504) in all test wells at 1:40,000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Cat. No. 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Immunohistochemistry analysis of Tau showing staining in the cytoplasm of paraffin-embedded human astroglioma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with Tau monoclonal antibody (TAU-5) (Cat. No. AHB0042) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Annotated product references
Cat. No. M701B was used in ELISA to study the role of human melanoma cell Wnt/beta-catenin signaling in immune suppression and resistance. Yaguchi T, Goto Y, Kido K et al. (2012) Immune suppression and resistance mediated by constitutive activation of Wnt/β-catenin signaling in human melanoma cells. J Immunol 185:2110–2117.
Cat. No. M701B was used in ELISA to develop and validate novel bispecific antibodies that activate and direct T cells against tumor cell. Cui H, Thomas JS, Burke TR Jr et al. (2012) Chemically programmed bispecific antibodies that recruit and activate T cells. J Biol Chem 287:28206–28214.
Cat. No. MA1-046 was used in western blot to characterize a novel hypomorphic mutation on chromosome 10 that affects the expression of Ostm1. Bosman EA, Estabel J, Ismail O et al. (2013) Omi, a recessive mutation on chromosome 10, is a novel allele of Ostm1. Mamm Genome 24:44–53.
Cat. No. MA1-046 was used in immunohistochemistry to study filamin A and its anatomical and subcellular localization in the brain of mature rats. Noam Y, Phan L, McClelland S et al. (2012) Distinct regional and subcellular localization of the actin-binding protein filamin A in the mature rat brain. J Comp Neurol 520:3013–3034.
For Research Use Only. Not for use in diagnostic procedures.