Optimized, automated assays for HCS
This automated assay enables quantitation and correlation of neuron and neurite morphology. Analysis can range from simple neurite outgrowth measurement to complex analysis of extended neurite outgrowth and branching. This can be combined with correlative studies on synaptic function as determined by the co-localization of pre- and postsynaptic markers (see Synaptogenesis Assay). Neurons are selected using a nuclear stain to locate the cell and a choice of neuron-specific stains or immunofluorescent labels to detect the cell body and neurite.