Optimized, automated assays for HCS
Generation of reactive oxygen species (ROS) is inevitable for aerobic organisms and, in healthy cells, occurs at a controlled rate. Increased production of ROS by a cell is an early hallmark of cellular stress. Intracellular ROS cause damage to proteins, nucleic acids, and membrane lipids. Oxidative damage of these biomolecules is associated with aging as well as a variety of pathological events, including atherosclerosis, carcinogenesis, ischemia reperfusion injury, and neurodegenerative disorders. Using fluorogenic reporters such as CellROX dyes allows for the real-time analysis of ROS generation in living cells and in fixed-cell preparations.