Qdot 800 probe is an excellent near-IR–fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~800 nm, Qdot 800 probe can be excited using any laser line from 350 nm up to 633 nm or a standard Cy5.5 filter. Optimum imaging results with brighter signals are achieved with a Qdot 800 filter or light cube.
Made from nanocrystals of semiconductor material, Qdot probes are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. In general they have an extremely broad excitation range and narrow emission with a large Stokes shift. Optimal brightness is generally achieved using a specific Qdot filter.