Gibco Cell Culture Bags
- Gibco media conveniently pre-filled in bags in a variety of formulations and sizes
- Specifically designed to increase productivity and reduce costs
- Easily integrates with most systems: universal design provides multiple connection options
- Gibco quality and reliability in a disposable format up to 1,000 L
Gibco flexible packaging options
Gibco PE bags are available prefilled with a selection of Gibco media and reagents in 5, 10 and 20 L sizes.
The Cellbag™ Bioreactor from Wave Biotech (part of GE Healthcare) is available prefilled with Gibco Sf-900 II Medium.
Overview of the product contact layer of Gibco universal bags
The new product contact layer of the Gibco universal bag is now ultralow density polyethylene and has the following benefits:
- Robust, clear and animal-origin free film
- Single-ply co-extruded film structure
- Extremely low extractable and leachable profile
- Superior gas transmission barrier properties
- Consistent film across every bag size (1–1,000 L)
All bags were transitioned to the new Gibco PE film during 2010.
|Physical Property*||Dimension||Value (Before / after gamma sterilization)||Procedure|
|Haze||%||5 / 5||ASTM D-1003|
|Clarity||%||98 / 98||ASTM D-1003|
|Transmittance||%||93 / 93||ASTM D-1003|
|Tensile strength at break||MPa||14 / 13||ASTM D-882|
|Elongation at break||%||280 / 300||ASTM D-882|
|Elastic modulus||MPa||370 / 350||ASTM D-882|
|Break at cold temperature||ºC||Below -45 / below -45||ISO 8570|
|Water vapor transmission rate**||g/(m2.day)||0.35 / 0.32||ASTM F-1249|
|Oxygen permeability**||cm3/(m2.day.atm)||< 0.05 / <0.05||ASTM D-3985|
|Carbon dioxide permeability**||cm3/(m2.day.atm)||<0.2 / <0.2||ASTM F-2476|
|Film thickness||0.325 mm|
|Gamma sterilization dose 25 kGy except **50 kGy|
|Appearance of solution S1
|Acidity or alkalinity
||≤ 1.5 mL 0.01 N NaOH
≤ 1.0 mL 0.01 N HCl
|Pass (0.60 mL)
Pass (0.40 mL)
|UV absorbance (220-340 nm)
||≤ 0.5 mL
||Pass (0.20 mL)
||≤ 1 ppm
||≤ 0.1 ppm
||≤ 0.05 ppm
|Extractable heavy metals
||≤ 2.5 ppm
||≤ 1 ppm
||≤ 0.1 ppm
||≤ 1 ppm
||≤ 0.1 ppm
|Substances soluble in hexane
|Antioxidants (max. 3)
|Amides and stearates
||USP <661>||≤ 15 mg||Pass
|Residue on ignition
||USP <661>||≤ 5 mg
||< 2 ppm
||≤ 10 mL
|Systemic injection test
||USP <88>||Class VI
Gas transmission rates
|Lower is Better
The Gibco PE film demonstrates significantly lower gas transmission rates in comparison to other bags on the market.
Gibco PE film construction
Gibco PE film pH stability
Shelf life stability study over a 19 month period for CD-CHO, DMEM, and FreeStyle 293. Media packaged in the Gibco PE film remains within pH specification limits.
1. Why switch to a new film?
A: We have a culture of continuous improvement and innovation. Based on feedback from customers, we designed a bag portfolio using a consistent film across all sizes. We also achieved a new film superior in function to other commercially available films.
2. When will I start to receive bags with the new film?
A: We will begin production in the new film starting in February, 2010. You may continue to receive product with the original film until our stock is depleted. We expect to have all product in the new Gibco PE film by mid year.
A: The commercially available film is a very clear, multi-layered single web film with a polyethylene contact surface that can be made into a wide range of bag sizes (250mL – 1,000L). In addition, the film is a market leader with exceptional gas barrier properties with very low extractable and leachable results.
4. Does this mean there are changes to the formulations?
A: No. There is no change to the product formulation inside the bag and the new Gibco PE film will not affect media properties or effectiveness. All products are still manufactured at our Gibco production sites in Grand Island, NY or Paisley, Scotland.
A: The shape and design of the existing bag will remain constant. All tubing and fittings of the universal bags will remain in place to offer a wide range of connection options.
The outer package of the new bags has been improved to provide more support and structure for transportation and storage. Your bag will now ship in all brown, 100% recyclable, heavyweight corrugated box.
We have also added some additional formulas as stocked catalog products. We will now sell Freestyle 293 and CD CHO in the universal bags with the Gibco® PE film.
6. In what size package will my bags ship?
A: Each bag size will arrive in a specific heavyweight corrugated box designed for shipment
5L – 12” x 14.25” x 5.5”
10L – 13” x 23.5” 6.125”
20L – 15” x 24” x 6.125”
7. What sized bags are currently available?
A: Large size bags are currently available with a continued release of additional sizes throughout the year. We plan to start filling select formulas in the 5, 10, and 20 liter bags starting in Q1 2010 and will have a rolling launch of all catalog offered formulas.
8. Will you continue to support other films?
A: Yes. Although we feel that the Gibco PE film exceeds many of the technical properties of the other available films, we will continue to offer and support the other currently available films through custom orders.
A: No. All custom re-orders in the small bags will use the same film/bag as your original order. If you would like to switch to the new film, please let us know during the order process and we will accommodate your request.
A: Nothing. The catalog numbers will remain the same and you will continue to get your orders as usual. Each formula may be available in the new film at different times, but the change will occur automatically with no action needed on your part.
A: We can share with you our list of products on stability. However, we do not put all products on stability – only representative formulations.
Bag size flexibility: You will now be able to receive a wider range of bag sizes using the same film which makes scale up easier for you. The ability to scale up will allow you to receive different sized bags without you having to validate multiple films.
Documentation: The documentation for the Gibco PE film exceeds many others. It includes USP Class VI, leachable, extractable, gas permeation, biological and physical property to name a few.
Clarity: The clarity of this film is much better than multi-webbed films and most single web films.
Contact layer: The Gibco PE film has a polyethylene contact layer.
Find the information you need for successful cell culture—including application notes, videos & webinars, and the Gibco Education virtual labs—in the Cell Culture & Transfection Learning Center.