Recovery of Total RNA From a Wide Range of Cell Numbers and Sample Types
Recovery of total RNA from a wide range of cell numbers and sample types. (A) RNA was isolated from 25 cells to 2 x 106 cells (K562) in 8 replicate wells using the MagMAX™-96 Total RNA Isolation Kit. Equivalent volumes of the recovered RNA (4% of eluted volume) were used for qRT-PCR (10 µL reactions) targeting human RNA polymerase II mRNA. The percent CV among Ct values for the 8 replicate samples of each cell number input was less than 3%. (B) Analysis on an Agilent® 2100 Bioanalyzer showed highly intact RNA isolated from HeLa cells, mouse kidney, corn leaf, and corn kernels.
For research use only. Not for use in diagnostic procedures.