TaqMan MicroRNA Assays

Applied Biosystems™ TaqMan™ MicroRNA Assays are innovative tools for miRNA research—from isolation through discovery, profiling, quantitation, validation and functional analysis. There are now even more choices for detection of mature, biologically active miRNA, designed to support a growing range of miRNA applications. The new Applied Biosystems™ TaqMan Advanced miRNA Assays use ligation-based universal reverse transcription for a streamlined and highly sensitive workflow. The standard TaqMan MicroRNA Assays, which employ target-specific stem-loop reverse transcription primers for 3’ extended templates, continue to cover a range of species using standard TaqMan Assay–based real-time PCR.

Now available: TaqMan Advanced miRNA assays in card, plate, and OpenArray format

Enable highly sensitive and specific quantification of mature miRNAs using qPCR.  Find your affordable solution using the search tool below. 

Search for pre-designed arrays ›      Design your own custom array ›

Assay search tool - find & buy your TaqMan Assays:

New TaqMan Advanced miRNA Assays use universal reverse transcription and provide highly sensitive quantification.

Standard TaqMan MicroRNA Assays use a target-specific stem-loop reverse transcription primer.

TaqMan Pri-miRNA Assays detect and measure primary miRNAs from human, mouse, or rat species.

miRBase v.21 update

The content for our standard TaqMan MicroRNA Assays and the new TaqMan Advanced miRNA Assays is aligned with release 21 of the miRBase database. For a complete list of standard TaqMan MicroRNA Assays, TaqMan Advanced miRNA Assays, and other technical resources, please visit our TaqMan MicroRNA Assays resource page.


Additional miRNA assays by type

TaqMan Non-coding RNA Assays for reliable detection and quantitation of non-coding human, mouse, or rat transcripts longer than 200 nucleotides.

Custom assays to quantitate small RNA are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.

miRNA assays by format

Predesigned TaqMan Advanced miRNA 96-well Plates are now available in both fast and standard format.  In addition, custom 96-well plates containing TaqMan Advanced miRNA assays or the classic chemistry TaqMan miRNA assays are available with the TaqMan Custom Plating Service

Predesigned TaqMan Advanced miRNA array cards are now available, as well as custom TaqMan array cards containing TaqMan Advanced miRNA assays or the classic chemistry TaqMan miRNA assays that are ordered using our configurator tool.  TaqMan array cards are the ideal solution for profiling large numbers of miRNAs and samples with limited material (each reaction requires only 2 µL of sample).  TaqMan array cards are compatible with the 7900HT, ViiA 7, QuantStudio 7, and QuantStudio 12K Flex real-time PCR systems.

Order your TaqMan MicroRNA Assays pre-plated into 384-well plates with the TaqMan Custom Plating Service. Such plates are perfect for analyzing a large number of samples for a few target miRNAs or non-coding RNAs or for evaluating a few samples for a large number of targets.

These plates accommodate 3,072 reactions at once, delivering the high-throughput capabilities required for large profiling studies. Designed for use with the Applied Biosystems OpenArray Real-Time PCR System and the QuantStudio 12K Flex Real-Time PCR System.

QuantStudio 12K Flex Real-Time PCR System

Video: Find the right TaqMan MicroRNA Assay

It's never been easier to search over 23,000 TaqMan   MicroRNA Assays with the new Assay Search tool from   Thermo Fisher Scientific. Watch this short video to see   how easy it is to find the right TaqMan MicroRNA Assay.

Resources

TaqMan MicroRNA Assays FAQs
Product Bulletin: Quantitate microRNAs with the specificity and sensitivity of real-time PCR
Download complete list of TaqMan MicroRNA Assays
New Protocol for generating Custom Pools of RT primers
Brochure: TaqMan microRNA Assays

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